Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4367556..4368053 Replicon chromosome
Accession NZ_CP042901
Organism Escherichia coli strain CFSAN061762

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CA898_RS21190 Protein ID WP_001173343.1
Coordinates 4367556..4367729 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CA898_RS21195 Protein ID WP_000398619.1
Coordinates 4367757..4368053 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA898_RS21180 (4364226) 4364226..4365683 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
CA898_RS21185 (4365920) 4365920..4367437 + 1518 WP_001295074.1 lysine--tRNA ligase -
CA898_RS21190 (4367556) 4367556..4367729 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CA898_RS21195 (4367757) 4367757..4368053 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CA898_RS21200 (4368280) 4368280..4368552 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
CA898_RS21205 (4368564) 4368564..4368794 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
CA898_RS21210 (4368975) 4368975..4370606 + 1632 WP_001216477.1 sensor histidine kinase -
CA898_RS21215 (4370603) 4370603..4371322 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T136563 WP_001173343.1 NZ_CP042901:c4367729-4367556 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T136563 NZ_CP042901:c4367729-4367556 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT136563 WP_000398619.1 NZ_CP042901:c4368053-4367757 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT136563 NZ_CP042901:c4368053-4367757 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References