Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-doc/Doc-Phd |
| Location | 91177..91778 | Replicon | plasmid pCFSAN061763 |
| Accession | NZ_CP042900 | ||
| Organism | Escherichia coli strain CFSAN061763 | ||
Toxin (Protein)
| Gene name | doc | Uniprot ID | V0AJ64 |
| Locus tag | CA897_RS24080 | Protein ID | WP_001216034.1 |
| Coordinates | 91177..91557 (-) | Length | 127 a.a. |
Antitoxin (Protein)
| Gene name | phd | Uniprot ID | U9YQH9 |
| Locus tag | CA897_RS24085 | Protein ID | WP_001190712.1 |
| Coordinates | 91557..91778 (-) | Length | 74 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CA897_RS24045 (CA897_024400) | 86228..87160 | - | 933 | WP_000081352.1 | homocysteine S-methyltransferase | - |
| CA897_RS24050 (CA897_024405) | 87147..88550 | - | 1404 | WP_001373486.1 | S-methylmethionine permease | - |
| CA897_RS24055 (CA897_024410) | 88794..89774 | + | 981 | Protein_95 | IS5 family transposase | - |
| CA897_RS24060 (CA897_024415) | 89984..90319 | + | 336 | WP_169329198.1 | type I deoxyribonuclease HsdR | - |
| CA897_RS24065 (CA897_024420) | 90269..90682 | - | 414 | Protein_97 | integrase core domain-containing protein | - |
| CA897_RS24070 (CA897_024425) | 90687..90965 | - | 279 | Protein_98 | pdcB | - |
| CA897_RS24075 (CA897_024430) | 90993..91172 | - | 180 | WP_001513661.1 | hypothetical protein | - |
| CA897_RS24080 (CA897_024435) | 91177..91557 | - | 381 | WP_001216034.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin |
| CA897_RS24085 (CA897_024440) | 91557..91778 | - | 222 | WP_001190712.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| CA897_RS24090 (CA897_024445) | 92041..92738 | + | 698 | WP_226458177.1 | IS1-like element IS1A family transposase | - |
| CA897_RS24095 (CA897_024450) | 92925..93218 | - | 294 | WP_226438991.1 | biofilm development regulator YmgB/AriR family protein | - |
| CA897_RS24100 (CA897_024455) | 93522..93626 | - | 105 | Protein_104 | IS3 family transposase | - |
| CA897_RS24440 | 93653..93802 | - | 150 | Protein_105 | IS3 family transposase | - |
| CA897_RS24110 (CA897_024465) | 95016..95630 | + | 615 | WP_032214276.1 | acid-sensing system DNA-binding response regulator EvgA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / qnrS1 / blaCTX-M-15 | - | 1..117296 | 117296 | |
| - | inside | IScluster/Tn | - | - | 84194..94448 | 10254 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 127 a.a. Molecular weight: 13615.32 Da Isoelectric Point: 5.1514
>T136539 WP_001216034.1 NZ_CP042900:c91557-91177 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
Download Length: 381 bp
>T136539 NZ_CP042900:c91557-91177 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG
Antitoxin
Download Length: 74 a.a. Molecular weight: 8133.10 Da Isoelectric Point: 4.7708
>AT136539 WP_001190712.1 NZ_CP042900:c91778-91557 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
Download Length: 222 bp
>AT136539 NZ_CP056127:12187-12453 [Enterobacter asburiae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0AJ64 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CJB6 |