Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4460488..4460985 Replicon chromosome
Accession NZ_CP042899
Organism Escherichia coli strain CFSAN061763

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CA897_RS21325 Protein ID WP_001173343.1
Coordinates 4460488..4460661 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CA897_RS21330 Protein ID WP_000398619.1
Coordinates 4460689..4460985 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA897_RS21315 (4457158) 4457158..4458615 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
CA897_RS21320 (4458852) 4458852..4460369 + 1518 WP_001550472.1 lysine--tRNA ligase -
CA897_RS21325 (4460488) 4460488..4460661 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CA897_RS21330 (4460689) 4460689..4460985 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CA897_RS21335 (4461214) 4461214..4461486 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
CA897_RS21340 (4461498) 4461498..4461728 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
CA897_RS21345 (4461908) 4461908..4463539 + 1632 WP_001216477.1 sensor histidine kinase -
CA897_RS21350 (4463536) 4463536..4464255 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T136537 WP_001173343.1 NZ_CP042899:c4460661-4460488 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T136537 NZ_CP056126:c3925350-3925009 [Enterobacter asburiae]
ATGAAAACTTTACCTGCAACAACTCTGCGGGTGGTGAAGCCCTGCCTGTCACCCGTGGCTATCTGGAAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTAAGGAACGTGTTATACAGGAACATATCG
ATGCCGGGATCTCGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGGTTT
AGCTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTTGGTACGGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT136537 WP_000398619.1 NZ_CP042899:c4460985-4460689 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT136537 NZ_CP042899:c4460985-4460689 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References