Detailed information of TA system TA136536

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4271818..4272650
• Replicon: chromosome
• Accession: NZ_CP042899
• Organism: Escherichia coli strain CFSAN061763

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: CA897_RS20355
• Protein ID: WP_000854765.1
• Coordinates: 4271818..4272192 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: CA897_RS20360
• Protein ID: WP_001295723.1
• Coordinates: 4272282..4272650 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA897_RS20325 (4268296)	4268296..4268445	-	150	Protein_3992	hypothetical protein	-
CA897_RS20330 (4268551)	4268551..4268727	-	177	WP_001586605.1	DUF957 domain-containing protein	-
CA897_RS20335 (4268744)	4268744..4269154	-	411	Protein_3994	DUF5983 family protein	-
CA897_RS20340 (4269239)	4269239..4269985	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
CA897_RS20345 (4270000)	4270000..4271541	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
CA897_RS20350 (4271735)	4271735..4271821	-	87	Protein_3997	DUF5983 family protein	-
CA897_RS20355 (4271818)	4271818..4272192	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
CA897_RS20360 (4272282)	4272282..4272650	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CA897_RS20365 (4272813)	4272813..4273034	-	222	WP_000692345.1	DUF987 domain-containing protein	-
CA897_RS20370 (4273103)	4273103..4273579	-	477	WP_001354275.1	RadC family protein	-
CA897_RS20375 (4273595)	4273595..4274068	-	474	WP_001387789.1	antirestriction protein	-
CA897_RS20380 (4274234)	4274234..4274407	-	174	WP_001183321.1	hypothetical protein	-
CA897_RS20385 (4274407)	4274407..4275225	-	819	WP_001234716.1	DUF932 domain-containing protein	-
CA897_RS20390 (4275343)	4275343..4275538	-	196	Protein_4005	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T136536 WP_000854765.1 NZ_CP042899:c4272192-4271818 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T136536 NZ_CP042899:c4272192-4271818 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT136536 WP_001295723.1 NZ_CP042899:c4272650-4272282 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT136536 NZ_CP056126:c3699995-3699729 [Enterobacter asburiae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGACGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TGTTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52