Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3634720..3635338 Replicon chromosome
Accession NZ_CP042899
Organism Escherichia coli strain CFSAN061763

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CA897_RS17430 Protein ID WP_001291435.1
Coordinates 3635120..3635338 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CA897_RS17425 Protein ID WP_000344800.1
Coordinates 3634720..3635094 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA897_RS17415 (3629809) 3629809..3631002 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CA897_RS17420 (3631025) 3631025..3634174 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
CA897_RS17425 (3634720) 3634720..3635094 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CA897_RS17430 (3635120) 3635120..3635338 + 219 WP_001291435.1 HHA domain-containing protein Toxin
CA897_RS17435 (3635510) 3635510..3636061 + 552 WP_000102564.1 maltose O-acetyltransferase -
CA897_RS17440 (3636177) 3636177..3636647 + 471 WP_000136192.1 YlaC family protein -
CA897_RS17445 (3636805) 3636805..3636963 + 159 Protein_3428 EAL domain-containing protein -
CA897_RS17450 (3637018) 3637018..3637715 + 698 WP_225893468.1 IS1 family transposase -
CA897_RS17455 (3637731) 3637731..3639137 + 1407 Protein_3430 cyclic-guanylate-specific phosphodiesterase PdeB -
CA897_RS17460 (3639179) 3639179..3639532 - 354 WP_001550741.1 DUF1428 family protein -
CA897_RS17470 (3639911) 3639911..3640222 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T136532 WP_001291435.1 NZ_CP042899:3635120-3635338 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T136532 NZ_CP042899:3635120-3635338 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT136532 WP_000344800.1 NZ_CP042899:3634720-3635094 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT136532 NZ_CP042899:3634720-3635094 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References