Detailed information of TA system TA136531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3232780..3233485
• Replicon: chromosome
• Accession: NZ_CP042899
• Organism: Escherichia coli strain CFSAN061763

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: CA897_RS15660
• Protein ID: WP_000539521.1
• Coordinates: 3232780..3233166 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CA897_RS15665
• Protein ID: WP_001280945.1
• Coordinates: 3233156..3233485 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA897_RS15640 (3228784)	3228784..3229410	+	627	WP_001595548.1	glutathione S-transferase GstB	-
CA897_RS15645 (3229407)	3229407..3230522	-	1116	WP_001595547.1	aldose sugar dehydrogenase YliI	-
CA897_RS15650 (3230633)	3230633..3231016	-	384	WP_000497137.1	biofilm formation regulator BssR	-
CA897_RS15655 (3231229)	3231229..3232554	+	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CA897_RS15660 (3232780)	3232780..3233166	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA897_RS15665 (3233156)	3233156..3233485	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CA897_RS15670 (3233555)	3233555..3233797	-	243	Protein_3087	diguanylate cyclase	-
CA897_RS15675 (3233853)	3233853..3234550	+	698	WP_225893468.1	IS1 family transposase	-
CA897_RS15680 (3234566)	3234566..3235660	-	1095	Protein_3089	diguanylate cyclase	-
CA897_RS15685 (3235668)	3235668..3238016	-	2349	WP_001328220.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T136531 WP_000539521.1 NZ_CP042899:3232780-3233166 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T136531 NZ_CP042899:3232780-3233166 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT136531 WP_001280945.1 NZ_CP042899:3233156-3233485 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT136531 NZ_CP042899:3233156-3233485 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6