Detailed information of TA system TA136529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2583183..2583821
• Replicon: chromosome
• Accession: NZ_CP042899
• Organism: Escherichia coli strain CFSAN061763

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: CA897_RS12445
• Protein ID: WP_000813795.1
• Coordinates: 2583645..2583821 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CA897_RS12440
• Protein ID: WP_076797675.1
• Coordinates: 2583183..2583599 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA897_RS12420 (2578335)	2578335..2579276	-	942	WP_001251315.1	ABC transporter permease	-
CA897_RS12425 (2579277)	2579277..2580290	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
CA897_RS12430 (2580308)	2580308..2581453	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
CA897_RS12435 (2581698)	2581698..2583104	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
CA897_RS12440 (2583183)	2583183..2583599	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CA897_RS12445 (2583645)	2583645..2583821	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CA897_RS12450 (2584043)	2584043..2584273	+	231	WP_023910283.1	YncJ family protein	-
CA897_RS12455 (2584365)	2584365..2586326	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CA897_RS12460 (2586399)	2586399..2586935	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
CA897_RS12465 (2587027)	2587027..2588199	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T136529 WP_000813795.1 NZ_CP042899:c2583821-2583645 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T136529 NZ_CP042899:c2583821-2583645 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT136529 WP_076797675.1 NZ_CP042899:c2583599-2583183 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT136529 NZ_CP056123:2240953-2241213 [Moritella sp. 24]
ATGAGCCGAATGTTAGCTTGGACTGACGAAGCATGGAGTGATTACTTGTATTGGCAGGGTCAAGACAAGAAAACACTTAA
ACGAATTAATAGGTTAATCACAGATACTAAGCGTTCGGCATTCGAAGGGATTGGCAAACCTGAGCCTTTGAAAGAAAATC
TAGCTGGTTTTTGGTCACGTAGAATCGACGATACCAATAGGTTAGTTTATGCTGTTAATGACACACATTTAACGATTATT
TCTTGTCGTTATCACTATTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1