Detailed information of TA system TA136528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2481846..2483434
• Replicon: chromosome
• Accession: NZ_CP042899
• Organism: Escherichia coli strain CFSAN061763

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A829LC11
• Locus tag: CA897_RS12025
• Protein ID: WP_001551128.1
• Coordinates: 2482112..2483434 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: CA897_RS12020
• Protein ID: WP_001296726.1
• Coordinates: 2481846..2482112 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA897_RS12020 (2481846)	2481846..2482112	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
CA897_RS12025 (2482112)	2482112..2483434	+	1323	WP_001551128.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
CA897_RS12030 (2483634)	2483634..2484986	-	1353	WP_001551127.1	SIR2 family protein	-
CA897_RS12035 (2485201)	2485201..2486233	-	1033	Protein_2368	ISNCY family transposase	-
CA897_RS12040 (2486600)	2486600..2487499	-	900	WP_001551125.1	LysR family transcriptional regulator	-
CA897_RS12045 (2487638)	2487638..2487946	+	309	WP_001221046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2485201..2486019	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49270.88 Da
• Isoelectric Point: 8.2472

>T136528 WP_001551128.1 NZ_CP042899:2482112-2483434 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T136528 NZ_CP056123:970300-971001 [Moritella sp. 24]
TTGCTACAATTGCCAGATTGTGAACAAAAAGCTGTTTATAGGAAAATAATGAGCTACGACTACTCTCAAAGTATCAATCC
AAAAAAAGCACTTATATGGCGTATTGTCCACCGTGATAATTTACCTTGGATTTTCGCTAATGGTTTGCATAGTGGTAATA
GCTCAACACAGTCAAAAAAATGGGTGCATATTGGTAACCCTGAGTTAACTAGCAAGCGGGCCTCGCATCCAGTACCTATA
GTGCCAAACGGTTTTTTAAATGATTATGTGCCATTTTATTTCACTCCATTTTCGGTCATGCTGAAAAACATTCATGGCGG
TAGAGGTGGTGTTAAACAACGCCATAATGATGAGATCGTAATCCTTGTATCGAGTGTTCACAAAATTAAAAATCTAGGGC
TAGACTTTGTTTTTACAGATAGCCATGCTTATTATCGTTGGGCTACTTACTATAATGAAGTTAAAGATCTGGATAAAATA
GATTGGTCTTTATTGCAGCAGCGGGATTTCAAAAGAGACGCGGATGATCCGGCCAAATTCGAGCGCTATCAAGCTGAAGC
ATTAGTACATGGATCTTGTCCTATTGACGCGCTTTTAGGCATTATTTGTTATTCAGAAAAAACCAAAACAGATATTGATA
AATTACTGATAGAAAGAAATATGAATTTAGATGTTTATGCTAGAACGGGATGGTACTTCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT136528 WP_001296726.1 NZ_CP042899:2481846-2482112 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT136528 NZ_CP042899:2481846-2482112 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A829LC11