Detailed information of TA system TA136503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4256971..4257566
• Replicon: chromosome
• Accession: NZ_CP042896
• Organism: Escherichia coli strain CFSAN061771

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: CA902_RS20040
• Protein ID: WP_000239581.1
• Coordinates: 4256971..4257321 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: CA902_RS20045
• Protein ID: WP_001223213.1
• Coordinates: 4257315..4257566 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA902_RS20020 (4252425)	4252425..4253447	-	1023	WP_001298067.1	ABC transporter permease	-
CA902_RS20025 (4253461)	4253461..4254963	-	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
CA902_RS20030 (4255096)	4255096..4256052	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
CA902_RS20035 (4256362)	4256362..4256892	+	531	WP_000055075.1	inorganic diphosphatase	-
CA902_RS20040 (4256971)	4256971..4257321	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
CA902_RS20045 (4257315)	4257315..4257566	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
CA902_RS20050 (4257778)	4257778..4258119	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
CA902_RS20055 (4258122)	4258122..4261901	-	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T136503 WP_000239581.1 NZ_CP042896:c4257321-4256971 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T136503 NZ_CP042896:c4257321-4256971 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT136503 WP_001223213.1 NZ_CP042896:c4257566-4257315 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT136503 NZ_CP042896:c4257566-4257315 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7