Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4007836..4008386 Replicon chromosome
Accession NZ_CP042896
Organism Escherichia coli strain CFSAN061771

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag CA902_RS18985 Protein ID WP_001160966.1
Coordinates 4007836..4008150 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag CA902_RS18990 Protein ID WP_000125568.1
Coordinates 4008153..4008386 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA902_RS18960 (4003511) 4003511..4004797 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
CA902_RS18965 (4004797) 4004797..4005786 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CA902_RS18970 (4005783) 4005783..4006604 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CA902_RS18975 (4006607) 4006607..4006984 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CA902_RS18980 (4006991) 4006991..4007839 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CA902_RS18985 (4007836) 4007836..4008150 - 315 WP_001160966.1 CcdB family protein Toxin
CA902_RS18990 (4008153) 4008153..4008386 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CA902_RS18995 (4008472) 4008472..4008951 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
CA902_RS19000 (4009143) 4009143..4011005 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
CA902_RS19005 (4010998) 4010998..4011528 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CA902_RS19010 (4011636) 4011636..4012967 - 1332 WP_001183201.1 MFS transporter -
CA902_RS19015 (4013025) 4013025..4013312 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T136502 WP_001160966.1 NZ_CP042896:c4008150-4007836 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T136502 NZ_CP056114:3274968-3275141 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT136502 WP_000125568.1 NZ_CP042896:c4008386-4008153 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT136502 NZ_CP056114:3274644-3274940 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References