Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3574871..3575489 Replicon chromosome
Accession NZ_CP042896
Organism Escherichia coli strain CFSAN061771

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CA902_RS16945 Protein ID WP_001291435.1
Coordinates 3575271..3575489 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CA902_RS16940 Protein ID WP_000344800.1
Coordinates 3574871..3575245 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA902_RS16930 (3569960) 3569960..3571153 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CA902_RS16935 (3571176) 3571176..3574325 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
CA902_RS16940 (3574871) 3574871..3575245 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CA902_RS16945 (3575271) 3575271..3575489 + 219 WP_001291435.1 HHA domain-containing protein Toxin
CA902_RS16950 (3575661) 3575661..3576212 + 552 WP_000536391.1 maltose O-acetyltransferase -
CA902_RS16955 (3576328) 3576328..3576798 + 471 WP_022645280.1 YlaC family protein -
CA902_RS16960 (3576962) 3576962..3578512 + 1551 WP_012602175.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CA902_RS16965 (3578553) 3578553..3578906 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CA902_RS16975 (3579285) 3579285..3579596 + 312 WP_000409911.1 MGMT family protein -
CA902_RS16980 (3579627) 3579627..3580199 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T136500 WP_001291435.1 NZ_CP042896:3575271-3575489 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T136500 NZ_CP042896:3575271-3575489 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT136500 WP_000344800.1 NZ_CP042896:3574871-3575245 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT136500 NZ_CP042896:3574871-3575245 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References