Detailed information of TA system TA136497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2857949..2858733
• Replicon: chromosome
• Accession: NZ_CP042896
• Organism: Escherichia coli strain CFSAN061771

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: CA902_RS13585
• Protein ID: WP_000613626.1
• Coordinates: 2858239..2858733 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: CA902_RS13580
• Protein ID: WP_139352217.1
• Coordinates: 2857949..2858242 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA902_RS13570 (2853098)	2853098..2854057	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
CA902_RS13575 (2854630)	2854630..2857815	+	3186	WP_077516769.1	ribonuclease E	-
CA902_RS13580 (2857949)	2857949..2858242	+	294	WP_139352217.1	DUF1778 domain-containing protein	Antitoxin
CA902_RS13585 (2858239)	2858239..2858733	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
CA902_RS13590 (2858828)	2858828..2859781	-	954	WP_001212763.1	flagellar hook-associated protein FlgL	-
CA902_RS13595 (2859793)	2859793..2861436	-	1644	WP_077516765.1	flagellar hook-associated protein FlgK	-
CA902_RS13600 (2861502)	2861502..2862443	-	942	WP_012311956.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
CA902_RS13605 (2862443)	2862443..2863540	-	1098	WP_000589300.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T136497 WP_000613626.1 NZ_CP042896:2858239-2858733 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T136497 NZ_CP042896:2858239-2858733 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11139.76 Da
• Isoelectric Point: 10.5423

>AT136497 WP_139352217.1 NZ_CP042896:2857949-2858242 [Escherichia coli]
MPAANNIPARREFLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT136497 NZ_CP042896:2857949-2858242 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATTTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: V0T0H9