Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 113559..114160 Replicon plasmid pCFSAN061772_01
Accession NZ_CP042894
Organism Escherichia coli strain CFSAN061772

Toxin (Protein)


Gene name doc Uniprot ID A0A838AVV6
Locus tag CA901_RS22965 Protein ID WP_063501799.1
Coordinates 113780..114160 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag CA901_RS22960 Protein ID WP_001190712.1
Coordinates 113559..113780 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA901_RS22940 (CA901_023120) 109633..111180 - 1548 WP_220531966.1 type I restriction-modification system subunit M -
CA901_RS22945 (CA901_023125) 111206..112186 + 981 WP_032072169.1 IS5-like element ISKpn26 family transposase -
CA901_RS22950 (CA901_023135) 112747..112998 + 252 WP_094336865.1 DNA polymerase III subunit theta -
CA901_RS22955 (CA901_023140) 113097..113486 + 390 WP_016246560.1 S24 family peptidase -
CA901_RS22960 (CA901_023145) 113559..113780 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CA901_RS22965 (CA901_023150) 113780..114160 + 381 WP_063501799.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CA901_RS24690 (CA901_023155) 114165..114344 + 180 WP_001411052.1 hypothetical protein -
CA901_RS22970 (CA901_023160) 114372..115415 + 1044 WP_220531963.1 DUF968 domain-containing protein -
CA901_RS22975 (CA901_023165) 115669..115890 + 222 WP_001361625.1 host cell division inhibitor Icd-like protein -
CA901_RS22980 (CA901_023170) 115887..117005 + 1119 Protein_114 phage antirepressor KilAC domain-containing protein -
CA901_RS22985 (CA901_023175) 117038..117889 - 852 WP_000611656.1 phage repressor protein C1 -
CA901_RS22990 (CA901_023180) 118000..118209 - 210 WP_063501801.1 hypothetical protein -
CA901_RS22995 (CA901_023195) 118814..119035 + 222 WP_116439157.1 creatininase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qnrS11 / floR / aph(6)-Id / aph(3'')-Ib / sul2 / tet(A) / blaTEM-1B / aadA2 / dfrA12 - 1..141344 141344
- flank IS/Tn - - 111206..112186 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13616.39 Da        Isoelectric Point: 5.6343

>T136480 WP_063501799.1 NZ_CP042894:113780-114160 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGTATGEISVSTVAATLRRLYGSAE

Download         Length: 381 bp

>T136480 NZ_CP042894:113780-114160 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGACTGCGACTGG
CGAGATATCTGTATCTACTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT136480 WP_001190712.1 NZ_CP042894:113559-113780 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT136480 NZ_CP042894:113559-113780 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A838AVV6

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References