Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4199181..4199678 Replicon chromosome
Accession NZ_CP042893
Organism Escherichia coli strain CFSAN061772

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CA901_RS20305 Protein ID WP_001173343.1
Coordinates 4199181..4199354 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CA901_RS20310 Protein ID WP_000398619.1
Coordinates 4199382..4199678 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA901_RS20295 (4195851) 4195851..4197308 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
CA901_RS20300 (4197545) 4197545..4199062 + 1518 WP_001295074.1 lysine--tRNA ligase -
CA901_RS20305 (4199181) 4199181..4199354 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CA901_RS20310 (4199382) 4199382..4199678 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CA901_RS20315 (4199905) 4199905..4200177 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
CA901_RS20320 (4200189) 4200189..4200419 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
CA901_RS20325 (4200600) 4200600..4202231 + 1632 WP_001216477.1 sensor histidine kinase -
CA901_RS20330 (4202228) 4202228..4202947 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T136478 WP_001173343.1 NZ_CP042893:c4199354-4199181 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T136478 NZ_CP042893:c4199354-4199181 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT136478 WP_000398619.1 NZ_CP042893:c4199678-4199382 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT136478 NZ_CP042893:c4199678-4199382 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References