Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3433143..3433761 Replicon chromosome
Accession NZ_CP042893
Organism Escherichia coli strain CFSAN061772

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CA901_RS16655 Protein ID WP_001291435.1
Coordinates 3433543..3433761 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CA901_RS16650 Protein ID WP_000344800.1
Coordinates 3433143..3433517 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA901_RS16640 (3428232) 3428232..3429425 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CA901_RS16645 (3429448) 3429448..3432597 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
CA901_RS16650 (3433143) 3433143..3433517 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CA901_RS16655 (3433543) 3433543..3433761 + 219 WP_001291435.1 HHA domain-containing protein Toxin
CA901_RS16660 (3433933) 3433933..3434484 + 552 WP_000102564.1 maltose O-acetyltransferase -
CA901_RS16665 (3434600) 3434600..3435070 + 471 WP_000136192.1 YlaC family protein -
CA901_RS16670 (3435234) 3435234..3436784 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CA901_RS16675 (3436826) 3436826..3437179 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CA901_RS16685 (3437558) 3437558..3437869 + 312 WP_000409911.1 MGMT family protein -
CA901_RS16690 (3437900) 3437900..3438472 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T136473 WP_001291435.1 NZ_CP042893:3433543-3433761 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T136473 NZ_CP042893:3433543-3433761 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT136473 WP_000344800.1 NZ_CP042893:3433143-3433517 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT136473 NZ_CP042893:3433143-3433517 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References