Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2271127..2271624 Replicon chromosome
Accession NZ_CP042892
Organism Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FVP48_RS13675 Protein ID WP_001173343.1
Coordinates 2271127..2271300 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FVP48_RS13680 Protein ID WP_000398619.1
Coordinates 2271328..2271624 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVP48_RS13665 2267797..2269254 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
FVP48_RS13670 2269491..2271008 + 1518 WP_001295074.1 lysine--tRNA ligase -
FVP48_RS13675 2271127..2271300 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FVP48_RS13680 2271328..2271624 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FVP48_RS13685 2271851..2272123 - 273 WP_000405651.1 N-acetyltransferase -
FVP48_RS13690 2272135..2272365 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
FVP48_RS13695 2272546..2274177 + 1632 WP_001216477.1 sensor histidine kinase -
FVP48_RS13700 2274174..2274893 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T136442 WP_001173343.1 NZ_CP042892:c2271300-2271127 [Escherichia coli O10:H32]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T136442 NZ_CP042892:c2271300-2271127 [Escherichia coli O10:H32]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT136442 WP_000398619.1 NZ_CP042892:c2271624-2271328 [Escherichia coli O10:H32]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT136442 NZ_CP056073:4062605-4063378 [Escherichia coli]
ATGGCATCAAAATTAAACTGGCTGTTGCAAAACACCTCTCCGGGAAGCCTGGTGTTACAATCGTGGCTGACCCGGAACAA
CATATCACCTTCTCTGGCTTTTAAATACGCGCAAAATGGCTGGTTAATAAAACTGCGGGCTGGCGTTTATGCGCGCGCAG
GACGAGAACCAGACTGGAGTGATGCGCTATGGTGCCTACAAAACCAACTTGAAGCGCCGGTGCATCTGGCCGGACTGAGT
AGTCTCTCCTGGCAGGGCCGTTCCCATTACCTACAACTTAAGCAGAACCAATGCTGGCTTAGCGTTGAAAACAAAGCGAT
ATTGCCAAAATGGTTTAAAGAATTTCCCGGCGTTGAGTGGTTGATGGTATCGGCCCTGAAGCTCCCTGACCTGGATGAAA
AATACCGAACTGAGCTTGAGGTTAAAGGGAAAAGAATTACCGCCAGCACGCCAGAACTGGCTGCCTATGAGCTGTTAAGC
GCAGTCCCTCAGGAAATCTCATTTGAGCACGCTGCCGAGCTTTTTCAGGGACTAGTCAATCTCAACCCACGTAAAGTGGA
GAAGCTGCTTACACTCAGTCAGTCCGTACAAACCAAGCGCCTGTATCTGTTTTTTGCCAGTTTCTATGAACATGCATGGT
TCAAACGCATAGATACCAGCAAAATCAACCTGGGATCGGGCAACCGACAAATCGTTGTTAATGGCAAGCTGAACACGCAA
TACCAGATAACTGTACCCGAAAATTTTACTAAGAAGGGAATAAGCCATGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References