Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2064719..2065295 Replicon chromosome
Accession NZ_CP042892
Organism Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag FVP48_RS12665 Protein ID WP_001295743.1
Coordinates 2065008..2065295 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag FVP48_RS12660 Protein ID WP_000063148.1
Coordinates 2064719..2065021 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVP48_RS12645 2061315..2063465 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
FVP48_RS12650 2063515..2063718 + 204 WP_000467859.1 YbdD/YjiX family protein -
FVP48_RS12655 2063729..2064685 + 957 WP_001295745.1 GTPase -
FVP48_RS12660 2064719..2065021 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
FVP48_RS12665 2065008..2065295 - 288 WP_001295743.1 BrnT family toxin Toxin
FVP48_RS12670 2065492..2066658 + 1167 WP_000800831.1 restriction endonuclease -
FVP48_RS12675 2066722..2068341 + 1620 WP_001029745.1 type I restriction-modification system subunit M -
FVP48_RS12680 2068331..2069635 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T136441 WP_001295743.1 NZ_CP042892:c2065295-2065008 [Escherichia coli O10:H32]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T136441 NZ_CP056073:3695727-3696005 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT136441 WP_000063148.1 NZ_CP042892:c2065021-2064719 [Escherichia coli O10:H32]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT136441 NZ_CP042892:c2065021-2064719 [Escherichia coli O10:H32]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References