Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1497403..1498021 Replicon chromosome
Accession NZ_CP042892
Organism Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FVP48_RS09810 Protein ID WP_001291435.1
Coordinates 1497803..1498021 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FVP48_RS09805 Protein ID WP_000344800.1
Coordinates 1497403..1497777 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVP48_RS09795 1492492..1493685 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FVP48_RS09800 1493708..1496857 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FVP48_RS09805 1497403..1497777 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FVP48_RS09810 1497803..1498021 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FVP48_RS09815 1498193..1498744 + 552 WP_000102564.1 maltose O-acetyltransferase -
FVP48_RS09820 1498860..1499330 + 471 WP_000136192.1 YlaC family protein -
FVP48_RS09825 1499494..1501044 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FVP48_RS09830 1501086..1501439 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FVP48_RS09840 1501818..1502129 + 312 WP_000409911.1 MGMT family protein -
FVP48_RS09845 1502160..1502732 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T136438 WP_001291435.1 NZ_CP042892:1497803-1498021 [Escherichia coli O10:H32]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T136438 NZ_CP042892:1497803-1498021 [Escherichia coli O10:H32]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT136438 WP_000344800.1 NZ_CP042892:1497403-1497777 [Escherichia coli O10:H32]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT136438 NZ_CP056073:3482973-3483347 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References