Detailed information of TA system TA136433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 7031..7532
• Replicon: plasmid pNMBU-W12E19_04
• Accession: NZ_CP042888
• Organism: Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: FVP48_RS01985
• Protein ID: WP_000702247.1
• Coordinates: 7031..7291 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: FVP48_RS01990
• Protein ID: WP_001132407.1
• Coordinates: 7281..7532 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVP48_RS01940	2083..2244	+	162	WP_001128311.1	hypothetical protein	-
FVP48_RS01945	2628..2789	+	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-
FVP48_RS01950	2939..3535	+	597	WP_000125165.1	hypothetical protein	-
FVP48_RS01955	3553..3723	-	171	WP_000186151.1	hypothetical protein	-
FVP48_RS01960	3911..4576	-	666	WP_000532148.1	P-loop NTPase	-
FVP48_RS01965	5174..5503	+	330	WP_000855120.1	hypothetical protein	-
FVP48_RS01970	6084..6407	+	324	WP_000427403.1	hypothetical protein	-
FVP48_RS01975	6449..6658	+	210	WP_000866036.1	hypothetical protein	-
FVP48_RS01980	6755..6979	+	225	WP_000814381.1	hypothetical protein	-
FVP48_RS01985	7031..7291	+	261	WP_000702247.1	hypothetical protein	Toxin
FVP48_RS01990	7281..7532	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
FVP48_RS01995	8082..8360	+	279	WP_000545938.1	hypothetical protein	-
FVP48_RS02000	8780..9046	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
FVP48_RS02005	9153..9335	-	183	WP_001481829.1	hypothetical protein	-
FVP48_RS02010	9893..10075	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
FVP48_RS02015	10100..10537	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
FVP48_RS02020	10668..10994	-	327	WP_000206697.1	hypothetical protein	-
FVP48_RS02025	11014..11721	-	708	WP_021503228.1	hypothetical protein	-
FVP48_RS02030	11723..12475	-	753	WP_000797966.1	conjugal transfer protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..57966	57966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T136433 WP_000702247.1 NZ_CP042888:7031-7291 [Escherichia coli O10:H32]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T136433 NZ_CP042888:7031-7291 [Escherichia coli O10:H32]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT136433 WP_001132407.1 NZ_CP042888:7281-7532 [Escherichia coli O10:H32]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT136433 NZ_CP042888:7281-7532 [Escherichia coli O10:H32]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0