Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1987..2534 Replicon plasmid pNMBU-W12E19_02
Accession NZ_CP042886
Organism Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)


Gene name parE Uniprot ID W0LWB3
Locus tag FVP48_RS00990 Protein ID WP_000970007.1
Coordinates 2253..2534 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag FVP48_RS00985 Protein ID WP_000079941.1
Coordinates 1987..2256 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVP48_RS00980 46..1077 + 1032 WP_000907869.1 replication initiation protein -
FVP48_RS00985 1987..2256 + 270 WP_000079941.1 hypothetical protein Antitoxin
FVP48_RS00990 2253..2534 + 282 WP_000970007.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FVP48_RS00995 2574..3422 + 849 WP_001057996.1 3'-5' exonuclease -
FVP48_RS01000 3539..4012 + 474 WP_001334658.1 hypothetical protein -
FVP48_RS01005 4489..4755 - 267 WP_000955263.1 hypothetical protein -
FVP48_RS01010 4755..5249 - 495 WP_001459505.1 hypothetical protein -
FVP48_RS01015 5305..5907 - 603 WP_000517694.1 hypothetical protein -
FVP48_RS01020 6278..6982 - 705 WP_001067858.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid qnrS1 / blaCTX-M-15 - 1..88772 88772


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10892.63 Da        Isoelectric Point: 9.7617

>T136424 WP_000970007.1 NZ_CP042886:2253-2534 [Escherichia coli O10:H32]
MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T136424 NZ_CP042886:2253-2534 [Escherichia coli O10:H32]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT136424 WP_000079941.1 NZ_CP042886:1987-2256 [Escherichia coli O10:H32]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT136424 NZ_CP056039:c2589-2338 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G1GDN5

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References