Detailed information of TA system TA136423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 60610..61145
• Replicon: plasmid pNMBU-W12E19_01
• Accession: NZ_CP042885
• Organism: Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q60228
• Locus tag: FVP48_RS00370
• Protein ID: WP_001270417.1
• Coordinates: 60610..60897 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FVP48_RS00375
• Protein ID: WP_173002174.1
• Coordinates: 60894..61145 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVP48_RS00335	55973..56664	+	692	Protein_66	PAS domain-containing protein	-
FVP48_RS00340	56752..56979	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
FVP48_RS00345	57013..57379	+	367	Protein_68	type IV conjugative transfer system pilin TraA	-
FVP48_RS00350	57394..57705	+	312	WP_000012106.1	type IV conjugative transfer system protein TraL	-
FVP48_RS00355	57727..58293	+	567	WP_000399780.1	type IV conjugative transfer system protein TraE	-
FVP48_RS00360	58280..59008	+	729	WP_173002173.1	type-F conjugative transfer system secretin TraK	-
FVP48_RS00365	59008..60543	+	1536	Protein_72	F-type conjugal transfer pilus assembly protein TraB	-
FVP48_RS00370	60610..60897	-	288	WP_001270417.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FVP48_RS00375	60894..61145	-	252	WP_173002174.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FVP48_RS00380	61318..61530	-	213	WP_032334873.1	hypothetical protein	-
FVP48_RS00385	61905..63110	+	1206	WP_173002175.1	AAA family ATPase	-
FVP48_RS00390	63107..64069	+	963	WP_173002176.1	ParB/RepB/Spo0J family partition protein	-
FVP48_RS00395	64227..65440	+	1214	WP_173002177.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	aap/aspU	1..167966	167966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11214.24 Da
• Isoelectric Point: 10.4703

>T136423 WP_001270417.1 NZ_CP042885:c60897-60610 [Escherichia coli O10:H32]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T136423 NZ_CP042885:c60897-60610 [Escherichia coli O10:H32]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGATAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9205.50 Da
• Isoelectric Point: 4.2108

>AT136423 WP_173002174.1 NZ_CP042885:c61145-60894 [Escherichia coli O10:H32]
MPNIILSDTSASVSELKKNPMATVNAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT136423 NZ_CP042885:c61145-60894 [Escherichia coli O10:H32]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAACGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGTTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Structures

Toxin

• AlphaFold DB: A0A5T8BSB4