Detailed information of TA system TA136418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 16224..16816
• Replicon: plasmid pNMBU-W12E19_01
• Accession: NZ_CP042885
• Organism: Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: FVP48_RS00110
• Protein ID: WP_000513532.1
• Coordinates: 16224..16472 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FVP48_RS00115
• Protein ID: WP_094171797.1
• Coordinates: 16469..16816 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVP48_RS00085	12075..12626	+	552	WP_000637611.1	hypothetical protein	-
FVP48_RS00090	12644..12814	-	171	WP_000186151.1	hypothetical protein	-
FVP48_RS00095	13007..13672	-	666	WP_000532148.1	P-loop NTPase	-
FVP48_RS00100	14290..14820	+	531	Protein_19	IS4-like element IS186B family transposase	-
FVP48_RS00105	14877..15574	+	698	Protein_20	IS1 family transposase	-
FVP48_RS00110	16224..16472	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
FVP48_RS00115	16469..16816	+	348	WP_094171797.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FVP48_RS00120	16904..17086	-	183	WP_173002225.1	hypothetical protein	-
FVP48_RS00125	17587..17886	+	300	WP_000466320.1	type II toxin-antitoxin system HicB family antitoxin	-
FVP48_RS00130	18147..18473	-	327	WP_173002159.1	hypothetical protein	-
FVP48_RS00135	19316..20164	+	849	WP_173002226.1	DUF4942 domain-containing protein	-
FVP48_RS00140	20249..20584	-	336	WP_000157095.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
FVP48_RS00145	20817..21149	+	333	WP_001291058.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	aap/aspU	1..167966	167966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T136418 WP_000513532.1 NZ_CP042885:16224-16472 [Escherichia coli O10:H32]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T136418 NZ_CP042885:16224-16472 [Escherichia coli O10:H32]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13176.99 Da
• Isoelectric Point: 4.9901

>AT136418 WP_094171797.1 NZ_CP042885:16469-16816 [Escherichia coli O10:H32]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAVTLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT136418 NZ_CP042885:16469-16816 [Escherichia coli O10:H32]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGTAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5