Detailed information of TA system TA136417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1768..2285
• Replicon: plasmid pNMBU-W12E19_01
• Accession: NZ_CP042885
• Organism: Escherichia coli O10:H32 strain NMBU-W12E19

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FVP48_RS00020
• Protein ID: WP_173002157.1
• Coordinates: 2052..2285 (+)
• Length: 78 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: FVP48_RS00015
• Protein ID: WP_001178087.1
• Coordinates: 1768..2052 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVP48_RS00010	1..858	+	858	WP_173002156.1	incFII family plasmid replication initiator RepA	-
FVP48_RS00015	1768..2052	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
FVP48_RS00020	2052..2285	+	234	WP_173002157.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FVP48_RS00025	2319..3016	+	698	Protein_4	IS1 family transposase	-
FVP48_RS00030	3156..3758	-	603	WP_173002158.1	proQ/FINO family protein	-
FVP48_RS00035	3934..4986	-	1053	WP_000655519.1	hypothetical protein	-
FVP48_RS00040	5199..5789	-	591	WP_001401589.1	hypothetical protein	-
FVP48_RS00045	6368..6529	+	162	WP_001128311.1	hypothetical protein	-
FVP48_RS00050	6723..6884	+	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	aap/aspU	1..167966	167966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-70)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 78 a.a.
• Molecular weight: 8910.46 Da
• Isoelectric Point: 9.6576

>T136417 WP_173002157.1 NZ_CP042885:2052-2285 [Escherichia coli O10:H32]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRVMLPTY

Nucleotide

• Length: 234 bp

>T136417 NZ_CP042885:2052-2285 [Escherichia coli O10:H32]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGGGTGATGCTGCCAACTTACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT136417 WP_001178087.1 NZ_CP042885:1768-2052 [Escherichia coli O10:H32]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT136417 NZ_CP042885:1768-2052 [Escherichia coli O10:H32]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: U9Y625