Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 173997..174640 Replicon plasmid pNMBU-W07E18_01
Accession NZ_CP042883
Organism Klebsiella pneumoniae strain NMBU-W07E18

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag FVP31_RS26730 Protein ID WP_014386165.1
Coordinates 174224..174640 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag FVP31_RS26725 Protein ID WP_032408893.1
Coordinates 173997..174227 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVP31_RS26700 169070..170065 - 996 WP_000611945.1 NAD(P)H-quinone oxidoreductase -
FVP31_RS26705 170072..171220 - 1149 WP_014386168.1 ABC transporter ATP-binding protein -
FVP31_RS26710 171391..172548 - 1158 WP_032408895.1 acetylornithine deacetylase -
FVP31_RS26715 172564..173238 - 675 WP_014386166.1 DUF1028 domain-containing protein -
FVP31_RS26720 173243..173677 - 435 WP_000103648.1 RidA family protein -
FVP31_RS26725 173997..174227 + 231 WP_032408893.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FVP31_RS26730 174224..174640 + 417 WP_014386165.1 PIN domain-containing protein Toxin
FVP31_RS26735 174963..175931 + 969 WP_074422984.1 IS5 family transposase -
FVP31_RS26740 176111..176485 - 375 WP_032408891.1 hypothetical protein -
FVP31_RS26745 176541..176867 - 327 WP_004152639.1 hypothetical protein -
FVP31_RS26750 176864..177592 - 729 WP_014386162.1 plasmid SOS inhibition protein A -
FVP31_RS26755 177589..178020 - 432 WP_014386161.1 conjugation system SOS inhibitor PsiB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 / blaTEM-1B - 1..194856 194856
- flank IS/Tn - - 174963..175931 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-130)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15127.44 Da        Isoelectric Point: 7.8841

>T136416 WP_014386165.1 NZ_CP042883:174224-174640 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Download         Length: 417 bp

>T136416 NZ_CP042883:174224-174640 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTAGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8854.89 Da        Isoelectric Point: 4.5112

>AT136416 WP_032408893.1 NZ_CP042883:173997-174227 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Download         Length: 231 bp

>AT136416 NZ_CP042883:173997-174227 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References