Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5237017..5237642 Replicon chromosome
Accession NZ_CP042882
Organism Klebsiella pneumoniae strain NMBU-W07E18

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FVP31_RS25430 Protein ID WP_002882817.1
Coordinates 5237017..5237400 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FVP31_RS25435 Protein ID WP_004150355.1
Coordinates 5237400..5237642 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVP31_RS25415 5234383..5235285 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FVP31_RS25420 5235282..5235917 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FVP31_RS25425 5235914..5236843 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FVP31_RS25430 5237017..5237400 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FVP31_RS25435 5237400..5237642 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FVP31_RS25440 5237847..5238764 + 918 WP_099761623.1 alpha/beta hydrolase -
FVP31_RS25445 5238779..5239720 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FVP31_RS25450 5239765..5240202 - 438 WP_099761625.1 D-tyrosyl-tRNA(Tyr) deacylase -
FVP31_RS25455 5240199..5241059 - 861 WP_004146232.1 virulence factor BrkB family protein -
FVP31_RS25460 5241053..5241652 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T136413 WP_002882817.1 NZ_CP042882:c5237400-5237017 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T136413 NZ_CP042882:c5237400-5237017 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT136413 WP_004150355.1 NZ_CP042882:c5237642-5237400 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT136413 NZ_CP042882:c5237642-5237400 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References