Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4801119..4801616 Replicon chromosome
Accession NZ_CP042878
Organism Escherichia coli strain NMBU_W05E18

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FVA71_RS23250 Protein ID WP_001173343.1
Coordinates 4801119..4801292 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FVA71_RS23255 Protein ID WP_000398619.1
Coordinates 4801320..4801616 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVA71_RS23240 4797789..4799246 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
FVA71_RS23245 4799483..4801000 + 1518 WP_001550472.1 lysine--tRNA ligase -
FVA71_RS23250 4801119..4801292 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FVA71_RS23255 4801320..4801616 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FVA71_RS23260 4801845..4802117 - 273 WP_000405647.1 N-acetyltransferase -
FVA71_RS23265 4802129..4802359 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
FVA71_RS23270 4802539..4804170 + 1632 WP_001216477.1 sensor histidine kinase -
FVA71_RS23275 4804167..4804886 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T136383 WP_001173343.1 NZ_CP042878:c4801292-4801119 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T136383 NZ_CP055883:c2760632-2760529 [Enterobacter sp. RHBSTW-00975]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT136383 WP_000398619.1 NZ_CP042878:c4801616-4801320 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT136383 NZ_CP042878:c4801616-4801320 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References