Detailed information of TA system TA136382

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4789335..4790170
• Replicon: chromosome
• Accession: NZ_CP042878
• Organism: Escherichia coli strain NMBU_W05E18

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: FVA71_RS23205
• Protein ID: WP_000854821.1
• Coordinates: 4789793..4790170 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FVA71_RS23200
• Protein ID: WP_032216526.1
• Coordinates: 4789335..4789703 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVA71_RS23160	4784458..4785342	+	885	WP_032216515.1	50S ribosome-binding GTPase	-
FVA71_RS23165	4785461..4786138	+	678	WP_032223228.1	hypothetical protein	-
FVA71_RS23170	4786144..4786377	+	234	WP_032289278.1	DUF905 family protein	-
FVA71_RS23175	4786476..4787294	+	819	WP_053902307.1	DUF945 domain-containing protein	-
FVA71_RS23180	4787386..4787871	+	486	WP_032216512.1	antirestriction protein	-
FVA71_RS23185	4787887..4788363	+	477	WP_001186724.1	RadC family protein	-
FVA71_RS23190	4788432..4788653	+	222	WP_001601167.1	DUF987 domain-containing protein	-
FVA71_RS23195	4788672..4789316	+	645	Protein_4539	antitoxin of toxin-antitoxin stability system	-
FVA71_RS23200	4789335..4789703	+	369	WP_032216526.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FVA71_RS23205	4789793..4790170	+	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FVA71_RS23210	4790167..4790316	+	150	Protein_4542	hypothetical protein	-
FVA71_RS23215	4790392..4790589	+	198	WP_114141299.1	DUF957 domain-containing protein	-
FVA71_RS23220	4790674..4791516	+	843	WP_032216511.1	DUF4942 domain-containing protein	-
FVA71_RS23225	4792265..4793803	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T136382 WP_000854821.1 NZ_CP042878:4789793-4790170 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T136382 NZ_CP042878:4789793-4790170 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13810.69 Da
• Isoelectric Point: 6.2066

>AT136382 WP_032216526.1 NZ_CP042878:4789335-4789703 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLLCTVTPCFGARLVQEGNRLHYLVDRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT136382 NZ_CP042878:4789335-4789703 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCTCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGTCGACCGCGCCGGCATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54