Detailed information of TA system TA136378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4495013..4495521
• Replicon: chromosome
• Accession: NZ_CP042878
• Organism: Escherichia coli strain NMBU_W05E18

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: FVA71_RS21725
• Protein ID: WP_001534843.1
• Coordinates: 4495312..4495521 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: FVA71_RS21720
• Protein ID: WP_000063161.1
• Coordinates: 4495013..4495315 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVA71_RS21705	4491541..4493691	+	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-
FVA71_RS21710	4493809..4494012	+	204	WP_001534846.1	YbdD/YjiX family protein	-
FVA71_RS21715	4494023..4494979	+	957	WP_001534845.1	GTPase	-
FVA71_RS21720	4495013..4495315	-	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
FVA71_RS21725	4495312..4495521	-	210	WP_001534843.1	BrnT family toxin	Toxin
FVA71_RS21730	4495617..4496783	+	1167	WP_001534841.1	restriction endonuclease	-
FVA71_RS21735	4496847..4497893	+	1047	Protein_4252	SAM-dependent DNA methyltransferase	-
FVA71_RS21740	4497957..4498661	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FVA71_RS21745	4498695..4499945	-	1251	WP_173009534.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4497957..4498661	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T136378 WP_001534843.1 NZ_CP042878:c4495521-4495312 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T136378 NZ_CP042878:c4495521-4495312 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT136378 WP_000063161.1 NZ_CP042878:c4495315-4495013 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT136378 NZ_CP042878:c4495315-4495013 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3