Detailed information of TA system TA136376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4135241..4136930
• Replicon: chromosome
• Accession: NZ_CP042878
• Organism: Escherichia coli strain NMBU_W05E18

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: FVA71_RS20020
• Protein ID: WP_000365809.1
• Coordinates: 4135241..4136161 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: FVA71_RS20025
• Protein ID: WP_001550661.1
• Coordinates: 4136148..4136930 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVA71_RS20005	4131331..4132458	-	1128	WP_001550663.1	MFS transporter	-
FVA71_RS20010	4132600..4133658	-	1059	WP_122987197.1	alpha/beta hydrolase	-
FVA71_RS20015	4133923..4134807	+	885	WP_001119037.1	LysR family transcriptional regulator	-
FVA71_RS20020	4135241..4136161	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
FVA71_RS20025	4136148..4136930	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
FVA71_RS20030	4137246..4140455	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
FVA71_RS20035	4140616..4140756	+	141	WP_001308408.1	ash family protein	-
FVA71_RS20040	4140802..4141272	+	471	WP_000201185.1	hypothetical protein	-
FVA71_RS20045	4141269..4141371	+	103	Protein_3925	helix-turn-helix domain-containing protein	-
FVA71_RS20050	4141463..4141621	+	159	Protein_3926	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T136376 WP_000365809.1 NZ_CP042878:c4136161-4135241 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T136376 NZ_CP055675:c4461563-4461411 [Escherichia fergusonii]
ATGCCGCAGAAATACGGATTACTTTCGTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGAGAATTATGAGCTGGCGGCGTTTTTAGCCTGCAAATTGAAAGAGTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT136376 WP_001550661.1 NZ_CP042878:c4136930-4136148 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT136376 NZ_CP042878:c4136930-4136148 [Escherichia coli]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GTCGTGAACCGGATTGGGTGGATGCGGTTTACTGCCTGCAATCGCAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGTCATGCGCACTATACCGAACAGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTTCTGCCTTTGAACAATCCGCAACGTTTGTCACGCTGTATACGTCAGGCCTTACCGCTGATGTAA
CGCCATTTACTACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTAATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGCCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: V0Z9M0