Detailed information of TA system TA136373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3234949..3235733
• Replicon: chromosome
• Accession: NZ_CP042878
• Organism: Escherichia coli strain NMBU_W05E18

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: FVA71_RS15800
• Protein ID: WP_000613626.1
• Coordinates: 3235239..3235733 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: FVA71_RS15795
• Protein ID: WP_001110447.1
• Coordinates: 3234949..3235242 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVA71_RS15785	3230099..3231058	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
FVA71_RS15790	3231631..3234816	+	3186	WP_001550951.1	ribonuclease E	-
FVA71_RS15795	3234949..3235242	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
FVA71_RS15800	3235239..3235733	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
FVA71_RS15805	3235828..3236781	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
FVA71_RS15810	3236793..3238436	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
FVA71_RS15815	3238502..3239443	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
FVA71_RS15820	3239443..3240540	-	1098	WP_001441925.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T136373 WP_000613626.1 NZ_CP042878:3235239-3235733 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T136373 NZ_CP042878:3235239-3235733 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT136373 WP_001110447.1 NZ_CP042878:3234949-3235242 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT136373 NZ_CP055675:4431249-4431303 [Escherichia fergusonii]
CAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6