Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1073286..1073940 Replicon chromosome
Accession NZ_CP042878
Organism Escherichia coli strain NMBU_W05E18

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag FVA71_RS05170 Protein ID WP_000244781.1
Coordinates 1073533..1073940 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0V9GG32
Locus tag FVA71_RS05165 Protein ID WP_001564007.1
Coordinates 1073286..1073552 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVA71_RS05145 1069374..1070807 - 1434 WP_001564009.1 6-phospho-beta-glucosidase BglA -
FVA71_RS05150 1070852..1071163 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
FVA71_RS05155 1071327..1071986 + 660 WP_000250274.1 hemolysin III family protein -
FVA71_RS05160 1072063..1073043 - 981 WP_089564224.1 tRNA-modifying protein YgfZ -
FVA71_RS05165 1073286..1073552 + 267 WP_001564007.1 FAD assembly factor SdhE Antitoxin
FVA71_RS05170 1073533..1073940 + 408 WP_000244781.1 protein YgfX Toxin
FVA71_RS05175 1073980..1074501 - 522 WP_001055874.1 flavodoxin FldB -
FVA71_RS05180 1074613..1075509 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FVA71_RS05185 1075534..1076244 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FVA71_RS05190 1076250..1077983 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T136356 WP_000244781.1 NZ_CP042878:1073533-1073940 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T136356 NZ_CP055601:18277-18558 [Escherichia fergusonii]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10565.15 Da        Isoelectric Point: 5.1599

>AT136356 WP_001564007.1 NZ_CP042878:1073286-1073552 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWMMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT136356 NZ_CP042878:1073286-1073552 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9GG32

References