Detailed information of TA system TA136351

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 74634..75468
• Replicon: chromosome
• Accession: NZ_CP042878
• Organism: Escherichia coli strain NMBU_W05E18

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8T5WFL4
• Locus tag: FVA71_RS00305
• Protein ID: WP_000854823.1
• Coordinates: 74634..75011 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8T5WFE1
• Locus tag: FVA71_RS00310
• Protein ID: WP_001285614.1
• Coordinates: 75100..75468 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVA71_RS00275	70276..71199	-	924	WP_000535949.1	carboxylate/amino acid/amine transporter	-
FVA71_RS00280	71310..72494	-	1185	WP_001172876.1	sugar efflux transporter	-
FVA71_RS00285	72881..73020	-	140	Protein_56	cytoplasmic protein	-
FVA71_RS00290	73288..74130	-	843	WP_173009490.1	DUF4942 domain-containing protein	-
FVA71_RS00295	74215..74412	-	198	WP_087890155.1	DUF957 domain-containing protein	-
FVA71_RS00300	74488..74637	-	150	Protein_59	hypothetical protein	-
FVA71_RS00305	74634..75011	-	378	WP_000854823.1	TA system toxin CbtA family protein	Toxin
FVA71_RS00310	75100..75468	-	369	WP_001285614.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FVA71_RS00315	75548..75769	-	222	WP_000692350.1	DUF987 domain-containing protein	-
FVA71_RS00320	75856..76332	-	477	WP_001186792.1	RadC family protein	-
FVA71_RS00325	76348..76827	-	480	WP_000706978.1	antirestriction protein	-
FVA71_RS00330	77093..77911	-	819	WP_114450809.1	DUF945 domain-containing protein	-
FVA71_RS00335	78001..78234	-	234	WP_000883181.1	DUF905 family protein	-
FVA71_RS00340	78240..78917	-	678	WP_001097562.1	hypothetical protein	-
FVA71_RS00345	79068..79748	-	681	WP_001278651.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	63587..95435	31848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.04 Da
• Isoelectric Point: 6.7093

>T136351 WP_000854823.1 NZ_CP042878:c75011-74634 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRMVNDISQGKYPEAKQ

Nucleotide

• Length: 378 bp

>T136351 NZ_CP042878:c75011-74634 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATGACATTAGCCAGGGGAAGTATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13596.35 Da
• Isoelectric Point: 6.0618

>AT136351 WP_001285614.1 NZ_CP042878:c75468-75100 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTSCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT136351 NZ_CP042878:c75468-75100 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GTCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA