Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 120770..121680 Replicon plasmid unnamed1
Accession NZ_CP042868
Organism Escherichia coli strain ATCC BAA-196

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag BAA196FS_RS23495 Protein ID WP_004181896.1
Coordinates 120770..121240 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag BAA196FS_RS23500 Protein ID WP_004181895.1
Coordinates 121237..121680 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAA196FS_RS23465 116266..116643 + 378 WP_060589151.1 hypothetical protein -
BAA196FS_RS24815 116841..117815 - 975 WP_004181899.1 hypothetical protein -
BAA196FS_RS23480 118418..118576 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
BAA196FS_RS23485 118648..119829 + 1182 WP_004883380.1 IS481 family transposase -
BAA196FS_RS23490 120400..120660 + 261 WP_004026352.1 hypothetical protein -
BAA196FS_RS23495 120770..121240 - 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
BAA196FS_RS23500 121237..121680 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
BAA196FS_RS23505 121781..123052 - 1272 WP_025368644.1 translesion error-prone DNA polymerase V subunit UmuC -
BAA196FS_RS23510 123052..123474 - 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
BAA196FS_RS23515 123560..125047 - 1488 WP_023332914.1 group II intron reverse transcriptase/maturase -
BAA196FS_RS23525 125738..126307 - 570 Protein_132 IS200/IS605 family element transposase accessory protein TnpB -
BAA196FS_RS23530 126309..126524 + 216 Protein_133 IS200/IS605 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaSCO-1 / aac(3)-IIa / blaTEM-10 / aph(3')-Ia / sul1 / qacE / ant(3'')-Ia / aac(6')-Ib / blaOXA-9 / blaTEM-1A / tet(B) / catA1 / dfrA8 - 1..279992 279992
- flank IS/Tn - - 118648..119829 1181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T136349 WP_004181896.1 NZ_CP042868:c121240-120770 [Escherichia coli]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T136349 NZ_CP055599:3369184-3369474 [Escherichia fergusonii]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACGAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT136349 WP_004181895.1 NZ_CP042868:c121680-121237 [Escherichia coli]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT136349 NZ_CP042868:c121680-121237 [Escherichia coli]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References