Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 131926..132836 Replicon plasmid unnamed1
Accession NZ_CP042866
Organism Escherichia coli strain ATCC BAA-196

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag BAA196NC_RS23545 Protein ID WP_004181896.1
Coordinates 131926..132396 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag BAA196NC_RS23550 Protein ID WP_004181895.1
Coordinates 132393..132836 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAA196NC_RS23515 127422..127799 + 378 WP_060589151.1 hypothetical protein -
BAA196NC_RS24420 127997..128971 - 975 WP_004181899.1 hypothetical protein -
BAA196NC_RS23530 129574..129732 - 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
BAA196NC_RS23535 129804..130985 + 1182 WP_004883380.1 IS481 family transposase -
BAA196NC_RS23540 131556..131816 + 261 WP_004026352.1 hypothetical protein -
BAA196NC_RS23545 131926..132396 - 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
BAA196NC_RS23550 132393..132836 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
BAA196NC_RS23555 132937..134208 - 1272 WP_025368644.1 translesion error-prone DNA polymerase V subunit UmuC -
BAA196NC_RS23560 134208..134630 - 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
BAA196NC_RS23565 134716..136203 - 1488 WP_023332914.1 group II intron reverse transcriptase/maturase -
BAA196NC_RS23575 136894..137463 - 570 Protein_139 transposase -
BAA196NC_RS23580 137465..137680 + 216 Protein_140 IS200/IS605 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaSCO-1 / aac(3)-IIa / blaTEM-10 / aph(3')-Ia / sul1 / qacE / ant(3'')-Ia / aac(6')-Ib / blaOXA-9 / blaTEM-1A / tet(B) / catA1 / dfrA8 - 1..266396 266396
- flank IS/Tn - - 129804..130985 1181
- flank IS/Tn - - 136894..137280 386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T136318 WP_004181896.1 NZ_CP042866:c132396-131926 [Escherichia coli]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T136318 NZ_CP042866:c132396-131926 [Escherichia coli]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT136318 WP_004181895.1 NZ_CP042866:c132836-132393 [Escherichia coli]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT136318 NZ_CP042866:c132836-132393 [Escherichia coli]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References