Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4212417..4212914 Replicon chromosome
Accession NZ_CP042865
Organism Escherichia coli strain ATCC BAA-196

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag BAA196NC_RS20645 Protein ID WP_001173343.1
Coordinates 4212417..4212590 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag BAA196NC_RS20650 Protein ID WP_000398619.1
Coordinates 4212618..4212914 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAA196NC_RS20635 4209087..4210544 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
BAA196NC_RS20640 4210781..4212298 + 1518 WP_001295074.1 lysine--tRNA ligase -
BAA196NC_RS20645 4212417..4212590 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
BAA196NC_RS20650 4212618..4212914 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
BAA196NC_RS20655 4213141..4213413 - 273 WP_000405651.1 N-acetyltransferase -
BAA196NC_RS20660 4213425..4213655 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
BAA196NC_RS20665 4213836..4215467 + 1632 WP_001216477.1 sensor histidine kinase -
BAA196NC_RS20670 4215464..4216183 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T136317 WP_001173343.1 NZ_CP042865:c4212590-4212417 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T136317 NZ_CP042865:c4212590-4212417 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT136317 WP_000398619.1 NZ_CP042865:c4212914-4212618 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT136317 NZ_CP055452:c200931-200692 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References