Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4798381..4799033 Replicon chromosome
Accession NZ_CP042858
Organism Klebsiella pneumoniae strain QD23

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag FU841_RS24370 Protein ID WP_017901321.1
Coordinates 4798381..4798806 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag FU841_RS24375 Protein ID WP_001261275.1
Coordinates 4798803..4799033 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FU841_RS24335 4794090..4794206 + 117 Protein_4686 transposase -
FU841_RS24340 4794331..4794501 - 171 Protein_4687 LysR family transcriptional regulator -
FU841_RS24345 4794626..4794739 + 114 WP_077252861.1 IS3 family transposase -
FU841_RS24350 4794831..4796402 - 1572 WP_049257520.1 sensor domain-containing diguanylate cyclase -
FU841_RS24360 4796800..4797360 - 561 Protein_4690 transposase -
FU841_RS24365 4797395..4798363 - 969 WP_077254762.1 IS5 family transposase -
FU841_RS24370 4798381..4798806 - 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
FU841_RS24375 4798803..4799033 - 231 WP_001261275.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FU841_RS24380 4799286..4801862 - 2577 WP_017901322.1 EstP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element aadA2 / ere(A) / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / aac(3)-IVa / aph(4)-Ia / sul2 / floR / blaCTX-M-65 / fosA3 / aph(3')-Ia / tet(C) / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / mph(A) / dfrA12 / blaTEM-1B / blaNDM-3 / aadA16 / dfrA27 - 4373245..4915543 542298
- flank IS/Tn - - 4797395..4798318 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T136263 WP_017901321.1 NZ_CP042858:c4798806-4798381 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T136263 NZ_CP042858:c4798806-4798381 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAAGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTGGCTTTGTTGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT136263 WP_001261275.1 NZ_CP042858:c4799033-4798803 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT136263 NZ_CP042858:c4799033-4798803 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References