Detailed information of TA system TA136260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4578617..4580339
• Replicon: chromosome
• Accession: NZ_CP042858
• Organism: Klebsiella pneumoniae strain QD23

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A0U2S2I2
• Locus tag: FU841_RS23195
• Protein ID: WP_064497040.1
• Coordinates: 4578617..4579555 (-)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A934MVJ4
• Locus tag: FU841_RS23200
• Protein ID: WP_000095577.1
• Coordinates: 4579548..4580339 (-)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FU841_RS23175	4574558..4575070	-	513	WP_175215521.1	S26 family signal peptidase	-
FU841_RS23180	4575054..4575401	-	348	WP_064497038.1	hypothetical protein	-
FU841_RS23185	4575394..4577793	-	2400	WP_064497039.1	type IV secretion system protein TraC	-
FU841_RS23190	4577793..4578485	-	693	WP_001228924.1	DsbC family protein	-
FU841_RS23195	4578617..4579555	-	939	WP_064497040.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
FU841_RS23200	4579548..4580339	-	792	WP_000095577.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
FU841_RS23205	4580560..4580946	-	387	WP_032469918.1	hypothetical protein	-
FU841_RS23210	4580943..4581515	-	573	WP_024008834.1	type IV conjugative transfer system lipoprotein TraV	-
FU841_RS23215	4581590..4582879	-	1290	WP_064497041.1	TraB/VirB10 family protein	-
FU841_RS23220	4582882..4583778	-	897	WP_064497055.1	type-F conjugative transfer system secretin TraK	-
FU841_RS23225	4583762..4584388	-	627	WP_064497042.1	pilus assembly protein	-
FU841_RS23230	4584385..4584666	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aadA2 / ere(A) / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / aac(3)-IVa / aph(4)-Ia / sul2 / floR / blaCTX-M-65 / fosA3 / aph(3')-Ia / tet(C) / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / mph(A) / dfrA12 / blaTEM-1B / blaNDM-3 / aadA16 / dfrA27	-	4373245..4915543	542298

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-272)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (99-250)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35542.81 Da
• Isoelectric Point: 4.9912

>T136260 WP_064497040.1 NZ_CP042858:c4579555-4578617 [Klebsiella pneumoniae]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIIVSSPVATIKIEVSPVARGTLHSAEIMAVQESVEYEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGVSREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQAEFDGMTFEKVECEDLASVRPMMLTSLQK
HFTERDYAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE

Nucleotide

• Length: 939 bp

>T136260 NZ_CP042858:c4579555-4578617 [Klebsiella pneumoniae]
ATGGATAAAGATAGCCCATATTACAAACAGGTTTCTTTGCTCATAAGAATGCTGCCTGTGGTAGCAACAGAGACGGTTTT
TGCACTTAAAGGTGGCACCGCCATTAATTTATTTGTGAGAGATTTTCCTCGGTTATCTGTAGATATTGATCTTGCTTATC
TTCCACTTGAACCAAGAGATGAGGCTTTGATTAATGTCAGGGCTGCATTGCAGCGCATTACAGACAGAATCAATACTCAA
CCAGATATCAGAGCGGCATTTCAGGATAATAAAGCTGATGAACTGAGAATAATTGTATCAAGTCCGGTTGCGACGATCAA
AATTGAAGTGTCGCCCGTCGCCAGAGGTACATTGCATAGTGCAGAAATAATGGCAGTTCAAGAGTCTGTTGAATACGAGT
TTGGTTATGCTGAGATTCAGGTAGTCAGTCTTCCCGATCTGTATGGTGGGAAATTGTGTGCTGCAATGGATCGCCAACAT
CCTCGCGACTTATTTGATGTGCGTATGTTACTTGGCAGTGAAGGGGTTTCGAGAGAGATTTTAGTGGGTTTTTTAACCTA
TACATTAAGTCATCCTCGGCCTATTAATGAAGTCATGTCGCCAAACTGGCAGCCGCTGAATGAGAAATTTCAGGCAGAAT
TTGATGGAATGACTTTTGAAAAAGTTGAATGCGAAGACTTAGCTTCTGTTAGGCCTATGATGTTAACTTCGCTGCAAAAA
CATTTCACAGAAAGGGATTATGCTTTCCTTATGTCATTCAAAAGAGGGCAACCTGACTGGGCATTGTTTGACTATCCTAA
TGCAGCAGACTTGCCAGCGATTCGTTGGAAGTTGCAGAACATAAACAAATTGGCAAAGAATCAAGCAAAACATCAAGAGC
AATTAGATAAATTGAAGCAAGTGCTTGATGATTGGCTTGTTAACGCAAACGCCGAGTAA

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30109.49 Da
• Isoelectric Point: 9.5097

>AT136260 WP_000095577.1 NZ_CP042858:c4580339-4579548 [Klebsiella pneumoniae]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLSSGVYYRPNAQGDIKPTWVDAIQALDVQLGVPVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

>AT136260 NZ_CP042858:c4580339-4579548 [Klebsiella pneumoniae]
ATGTCATCTAAAATAAACTGGCTTGTTGCTCATACTTCTCCTGGTGCGCTAGTACTTCAGCAATGGCTGACTGAAAACGG
CGTGAGCTACTCGTTGGCTCAAAAGTACGCGCAGAACGGTTGGCTGAAGAAGCTTAGTTCTGGTGTGTACTATCGTCCAA
ATGCGCAGGGCGATATAAAGCCAACTTGGGTTGATGCCATTCAAGCATTGGATGTGCAATTGGGCGTTCCAGTTCATTTG
GCTGGATTGAGCAGCTTAACTCACCAAGGACTGAGTCACTATTTGCAGCTGAACAAAGAGCAAGTTTGGATTTGCGTTAA
AAACAAGTCGTCCTTACCGAAATGGTTTCGTGAATTCCCTTATCAGAATTGGTTTTACTGCGGAAACCATAAGCTTGAAG
TGAATCCCGAGAAAGATTTGAAAAGGATCACGGTTAAAGAGAAAGAGCTCACTGTCAGCTGTGCAGAACTTGCTGCCTAT
GAAGTGGTAGATGCGATTGGAAAGCTGATTTCATTTGAGCATGTCGCAGAATTATTTCAGGGTTTAGTCAATCTTAGCCC
TAGAAAAGTACAAGATATTCTTGAACGAAGCAGCTCTGTTCAGGCAAACCGAATATTTCTATTTCTGGGTCGATACTACG
ATCACCAGTGGGTCAATCGCATAGATGAAACAAGAATTAAATTGGGGGCAGGAAAGCGGCAGGTTGTCGAAAAAGGACGT
TTTGATGAGCGATATCAAATCACAGTGCCAGAGATATTAAGCGTCAAAAAAGGTGAACAACATAATGGATAA

Structures

Toxin

• AlphaFold DB: A0A0U2S2I2