Detailed information of TA system TA136229

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2775831..2776662
• Replicon: chromosome
• Accession: NZ_CP042846
• Organism: Escherichia coli strain JME64

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: FVE20_RS13670
• Protein ID: WP_000854814.1
• Coordinates: 2776288..2776662 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: FVE20_RS13665
• Protein ID: WP_001285584.1
• Coordinates: 2775831..2776199 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE20_RS13650	2773498..2775030	+	1533	WP_001350525.1	hypothetical protein	-
FVE20_RS13655	2775027..2775473	+	447	WP_000187523.1	RadC family protein	-
FVE20_RS13660	2775536..2775757	+	222	WP_000692323.1	DUF987 domain-containing protein	-
FVE20_RS13665	2775831..2776199	+	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FVE20_RS13670	2776288..2776662	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FVE20_RS13675	2776659..2776853	+	195	WP_000988600.1	hypothetical protein	-
FVE20_RS22580	2776866..2776979	+	114	WP_001161660.1	DUF957 domain-containing protein	-
FVE20_RS13685	2777268..2777396	-	129	Protein_2627	transposase domain-containing protein	-
FVE20_RS13690	2777468..2777650	+	183	WP_001016348.1	hypothetical protein	-
FVE20_RS13695	2777751..2778080	-	330	WP_000450409.1	DUF496 family protein	-
FVE20_RS13700	2778252..2779310	-	1059	WP_001200891.1	FUSC family protein	-
FVE20_RS13705	2779508..2779981	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
FVE20_RS13710	2780100..2781266	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T136229 WP_000854814.1 NZ_CP042846:2776288-2776662 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T136229 NZ_CP042846:2776288-2776662 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT136229 WP_001285584.1 NZ_CP042846:2775831-2776199 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT136229 NZ_CP042846:2775831-2776199 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A1M2E8G6