Detailed information of TA system TA136206

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 964765..965444
• Replicon: chromosome
• Accession: NZ_CP042846
• Organism: Escherichia coli strain JME64

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: FVE20_RS04535
• Protein ID: WP_000854672.1
• Coordinates: 964765..965106 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: FVE20_RS04540
• Protein ID: WP_000070395.1
• Coordinates: 965127..965444 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE20_RS04510	960042..960443	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
FVE20_RS04515	960482..961537	-	1056	WP_000749863.1	phosphoporin PhoE	-
FVE20_RS04520	961825..962928	+	1104	WP_001285288.1	glutamate 5-kinase	-
FVE20_RS04525	962940..964193	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
FVE20_RS04535	964765..965106	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
FVE20_RS04540	965127..965444	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
FVE20_RS04545	965463..965684	-	222	WP_000691994.1	DUF987 domain-containing protein	-
FVE20_RS04550	965693..966169	-	477	WP_000811693.1	RadC family protein	-
FVE20_RS04555	966185..966643	-	459	WP_000211838.1	antirestriction protein	-
FVE20_RS04560	966741..966980	-	240	WP_000194654.1	DUF905 domain-containing protein	-
FVE20_RS04565	967057..967524	-	468	WP_001547765.1	hypothetical protein	-
FVE20_RS04570	967547..967990	-	444	WP_000824223.1	hypothetical protein	-
FVE20_RS04575	967990..968217	-	228	WP_001548158.1	hypothetical protein	-
FVE20_RS04580	968213..968404	-	192	Protein_871	DeoR family transcriptional regulator	-
FVE20_RS04585	968621..969442	-	822	WP_000197389.1	DUF945 domain-containing protein	-
FVE20_RS04590	969534..970397	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T136206 WP_000854672.1 NZ_CP042846:c965106-964765 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T136206 NZ_CP042846:c965106-964765 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT136206 WP_000070395.1 NZ_CP042846:c965444-965127 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT136206 NZ_CP042846:c965444-965127 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684