Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3743894..3744548 Replicon chromosome
Accession NZ_CP042845
Organism Escherichia coli strain JME65

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FVE21_RS18200 Protein ID WP_000244777.1
Coordinates 3743894..3744301 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FVE21_RS18205 Protein ID WP_000354046.1
Coordinates 3744282..3744548 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE21_RS18180 3739851..3741584 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
FVE21_RS18185 3741590..3742300 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FVE21_RS18190 3742325..3743221 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FVE21_RS18195 3743333..3743854 + 522 WP_001055874.1 flavodoxin FldB -
FVE21_RS18200 3743894..3744301 - 408 WP_000244777.1 toxin CptA Toxin
FVE21_RS18205 3744282..3744548 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FVE21_RS18210 3744791..3745771 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FVE21_RS18215 3745967..3746626 - 660 WP_000250274.1 hemolysin III family protein -
FVE21_RS18220 3746790..3747101 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
FVE21_RS18225 3747146..3748579 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
FVE21_RS18230 3748636..3749379 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T136191 WP_000244777.1 NZ_CP042845:c3744301-3743894 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T136191 NZ_CP042845:c3744301-3743894 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT136191 WP_000354046.1 NZ_CP042845:c3744548-3744282 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT136191 NZ_CP055277:c3249469-3249224 [Acinetobacter sp. NEB 394]
ATGAGCTTGGAAGAACGTAAAGTAATCTACCGTGCACGTCGTGGTTTAAAAGAAATTGATGTGTACTTTGACCCTTATGT
GAAAAATTATTATCTTCAGGCAGATCCTGCTGAAAAGGCCATGTTTGCGGCCTTGGTTGAACAGGAAGATCCAGATTTGC
TGGATTGGTTTATGGAAGTCTCTGAGCCGCCACGCCCTGAAATGAAAACCTTGATTCAAAAATTAAAGCATTATGTACAC
GGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References