Detailed information of TA system TA136163

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 969646..970325
• Replicon: chromosome
• Accession: NZ_CP042845
• Organism: Escherichia coli strain JME65

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: FVE21_RS04560
• Protein ID: WP_000854672.1
• Coordinates: 969646..969987 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: FVE21_RS04565
• Protein ID: WP_000070395.1
• Coordinates: 970008..970325 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE21_RS04535	964923..965324	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
FVE21_RS04540	965363..966418	-	1056	WP_000749863.1	phosphoporin PhoE	-
FVE21_RS04545	966706..967809	+	1104	WP_001285288.1	glutamate 5-kinase	-
FVE21_RS04550	967821..969074	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
FVE21_RS04560	969646..969987	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
FVE21_RS04565	970008..970325	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
FVE21_RS04570	970344..970565	-	222	WP_000691994.1	DUF987 domain-containing protein	-
FVE21_RS04575	970574..971050	-	477	WP_000811693.1	RadC family protein	-
FVE21_RS04580	971066..971524	-	459	WP_000211838.1	antirestriction protein	-
FVE21_RS04585	971622..971861	-	240	WP_000194654.1	DUF905 domain-containing protein	-
FVE21_RS04590	971938..972405	-	468	WP_001547765.1	hypothetical protein	-
FVE21_RS04595	972428..972871	-	444	WP_000824223.1	hypothetical protein	-
FVE21_RS04600	972871..973098	-	228	WP_001548158.1	hypothetical protein	-
FVE21_RS04605	973094..973285	-	192	Protein_876	DeoR family transcriptional regulator	-
FVE21_RS04610	973502..974323	-	822	WP_000197389.1	DUF945 domain-containing protein	-
FVE21_RS04615	974415..975278	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T136163 WP_000854672.1 NZ_CP042845:c969987-969646 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T136163 NZ_CP055259:3740355-3740633 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT136163 WP_000070395.1 NZ_CP042845:c970325-970008 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT136163 NZ_CP042845:c970325-970008 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684