Detailed information of TA system TA136143

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2780944..2781775
• Replicon: chromosome
• Accession: NZ_CP042844
• Organism: Escherichia coli strain JME66

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: FVE22_RS13700
• Protein ID: WP_000854814.1
• Coordinates: 2781401..2781775 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: FVE22_RS13695
• Protein ID: WP_001285584.1
• Coordinates: 2780944..2781312 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE22_RS13680	2778611..2780143	+	1533	WP_001350525.1	hypothetical protein	-
FVE22_RS13685	2780140..2780586	+	447	WP_000187523.1	RadC family protein	-
FVE22_RS13690	2780649..2780870	+	222	WP_000692323.1	DUF987 domain-containing protein	-
FVE22_RS13695	2780944..2781312	+	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FVE22_RS13700	2781401..2781775	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FVE22_RS13705	2781772..2781966	+	195	WP_000988600.1	hypothetical protein	-
FVE22_RS22610	2781979..2782092	+	114	WP_001161660.1	DUF957 domain-containing protein	-
FVE22_RS13715	2782381..2782509	-	129	Protein_2633	transposase domain-containing protein	-
FVE22_RS13720	2782581..2782763	+	183	WP_001016348.1	hypothetical protein	-
FVE22_RS13725	2782864..2783193	-	330	WP_000450409.1	DUF496 family protein	-
FVE22_RS13730	2783365..2784423	-	1059	WP_001200891.1	FUSC family protein	-
FVE22_RS13735	2784621..2785094	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
FVE22_RS13740	2785213..2786379	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T136143 WP_000854814.1 NZ_CP042844:2781401-2781775 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T136143 NZ_CP042844:2781401-2781775 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT136143 WP_001285584.1 NZ_CP042844:2780944-2781312 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT136143 NZ_CP042844:2780944-2781312 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6