Detailed information of TA system TA136102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 3474509..3475946
• Replicon: chromosome
• Accession: NZ_CP042843
• Organism: Escherichia coli strain JME67

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: FVE23_RS16915
• Protein ID: WP_000155570.1
• Coordinates: 3474509..3475582 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: FVE23_RS16920
• Protein ID: WP_000461704.1
• Coordinates: 3475575..3475946 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE23_RS16900	3469942..3472131	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
FVE23_RS16905	3472128..3473744	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
FVE23_RS16910	3474104..3474367	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
FVE23_RS16915	3474509..3475582	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
FVE23_RS16920	3475575..3475946	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
FVE23_RS16925	3476301..3477164	+	864	WP_000787597.1	GTPase family protein	-
FVE23_RS16930	3477256..3478077	+	822	WP_000197391.1	DUF945 domain-containing protein	-
FVE23_RS16935	3478294..3478995	+	702	WP_000189409.1	WYL domain-containing protein	-
FVE23_RS16940	3479036..3479272	+	237	WP_001144029.1	hypothetical protein	-
FVE23_RS16945	3479272..3479715	+	444	WP_000824222.1	hypothetical protein	-
FVE23_RS16950	3479739..3480206	+	468	WP_000702495.1	hypothetical protein	-
FVE23_RS16955	3480431..3480745	-	315	WP_000065803.1	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T136102 WP_000155570.1 NZ_CP042843:3474509-3475582 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T136102 NZ_CP055251:c2088518-2088324 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT136102 WP_000461704.1 NZ_CP042843:3475575-3475946 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT136102 NZ_CP042843:3475575-3475946 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4