Detailed information of TA system TA136064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 141204..141805
• Replicon: plasmid unnamed4
• Accession: NZ_CP042840
• Organism: Enterococcus sp. DA9

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0V7YM55
• Locus tag: FTX43_RS14655
• Protein ID: WP_002330364.1
• Coordinates: 141204..141548 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6B3Q8V0
• Locus tag: FTX43_RS14660
• Protein ID: WP_002318881.1
• Coordinates: 141542..141805 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FTX43_RS14625	136207..136717	-	511	Protein_142	hypothetical protein	-
FTX43_RS14630	136858..137208	-	351	WP_002318474.1	hypothetical protein	-
FTX43_RS14635	137201..138526	-	1326	WP_002318473.1	Y-family DNA polymerase	-
FTX43_RS14640	138770..139237	-	468	WP_147607153.1	hypothetical protein	-
FTX43_RS14645	139256..140131	-	876	WP_002318471.1	hypothetical protein	-
FTX43_RS14650	140181..141089	-	909	WP_113813388.1	IS982 family transposase	-
FTX43_RS14655	141204..141548	-	345	WP_002330364.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FTX43_RS14660	141542..141805	-	264	WP_002318881.1	PbsX family transcriptional regulator	Antitoxin
FTX43_RS14665	141992..142531	+	540	Protein_150	IS30 family transposase	-
FTX43_RS14675	142953..143195	-	243	WP_002318679.1	hypothetical protein	-
FTX43_RS14680	143077..143316	-	240	Protein_152	IS6 family transposase	-
FTX43_RS14685	143394..143999	-	606	Protein_153	IS30 family transposase	-
FTX43_RS14690	144126..145076	-	951	WP_002319255.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
FTX43_RS14695	145156..146109	-	954	WP_002319254.1	ribonucleotide-diphosphate reductase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	140181..143999	3818
	-	inside	Non-Mobilizable plasmid	-	-	1..159791	159791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13024.11 Da
• Isoelectric Point: 8.8971

>T136064 WP_002330364.1 NZ_CP042840:c141548-141204 [Enterococcus sp. DA9]
MVDVPRQGDILLLNTAPRSGHEQTGRRPYIVLSHDIIADTSNVVIVAPITTTSRNYPLYIEITENHKMKTRGKVMLDQIT
TIDYEARNCKFLEKASEKLIEELLTKIRAVFQKV

Nucleotide

• Length: 345 bp

>T136064 NZ_CP042840:c141548-141204 [Enterococcus sp. DA9]
ATGGTAGATGTTCCAAGACAAGGTGATATTCTTTTATTGAACACAGCTCCCAGATCTGGCCATGAACAGACTGGAAGAAG
ACCTTATATTGTTCTTAGTCACGATATTATTGCAGATACTAGTAATGTGGTTATTGTTGCACCGATAACTACAACTAGTA
GAAATTACCCACTATACATTGAAATAACAGAAAATCATAAAATGAAAACACGCGGAAAAGTTATGTTAGATCAAATCACA
ACAATTGATTATGAAGCCAGGAACTGCAAATTTCTAGAAAAAGCTTCTGAAAAATTAATAGAAGAATTATTAACAAAAAT
ACGAGCTGTATTTCAAAAAGTATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10071.69 Da
• Isoelectric Point: 5.2300

>AT136064 WP_002318881.1 NZ_CP042840:c141805-141542 [Enterococcus sp. DA9]
MEMIPIKKWGNSNGLRLPKNLMEFLELKTDDRVSIKSEDVNGKRRLIIEPAISKEEVTIEELFKDYTEGKVNIKIQDLGS
PVGRELW

Nucleotide

• Length: 264 bp

>AT136064 NZ_CP042840:c141805-141542 [Enterococcus sp. DA9]
ATGGAAATGATACCAATCAAAAAATGGGGAAATAGCAACGGACTTAGATTGCCAAAAAATTTAATGGAATTTCTGGAATT
AAAGACTGATGATCGAGTATCAATCAAAAGCGAAGATGTTAATGGAAAAAGACGATTAATTATCGAACCAGCAATTTCAA
AAGAAGAAGTAACTATTGAAGAGCTTTTCAAAGATTACACAGAGGGCAAAGTAAATATTAAAATTCAGGATTTAGGAAGC
CCAGTAGGGAGAGAACTATGGTAG

Structures

Toxin

• AlphaFold DB: A0A0V7YM55

Antitoxin

• AlphaFold DB: A0A6B3Q8V0