Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 141204..141805 Replicon plasmid unnamed4
Accession NZ_CP042840
Organism Enterococcus sp. DA9

Toxin (Protein)


Gene name mazF Uniprot ID A0A0V7YM55
Locus tag FTX43_RS14655 Protein ID WP_002330364.1
Coordinates 141204..141548 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6B3Q8V0
Locus tag FTX43_RS14660 Protein ID WP_002318881.1
Coordinates 141542..141805 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FTX43_RS14625 136207..136717 - 511 Protein_142 hypothetical protein -
FTX43_RS14630 136858..137208 - 351 WP_002318474.1 hypothetical protein -
FTX43_RS14635 137201..138526 - 1326 WP_002318473.1 Y-family DNA polymerase -
FTX43_RS14640 138770..139237 - 468 WP_147607153.1 hypothetical protein -
FTX43_RS14645 139256..140131 - 876 WP_002318471.1 hypothetical protein -
FTX43_RS14650 140181..141089 - 909 WP_113813388.1 IS982 family transposase -
FTX43_RS14655 141204..141548 - 345 WP_002330364.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FTX43_RS14660 141542..141805 - 264 WP_002318881.1 PbsX family transcriptional regulator Antitoxin
FTX43_RS14665 141992..142531 + 540 Protein_150 IS30 family transposase -
FTX43_RS14675 142953..143195 - 243 WP_002318679.1 hypothetical protein -
FTX43_RS14680 143077..143316 - 240 Protein_152 IS6 family transposase -
FTX43_RS14685 143394..143999 - 606 Protein_153 IS30 family transposase -
FTX43_RS14690 144126..145076 - 951 WP_002319255.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
FTX43_RS14695 145156..146109 - 954 WP_002319254.1 ribonucleotide-diphosphate reductase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 140181..143999 3818
- inside Non-Mobilizable plasmid - - 1..159791 159791


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13024.11 Da        Isoelectric Point: 8.8971

>T136064 WP_002330364.1 NZ_CP042840:c141548-141204 [Enterococcus sp. DA9]
MVDVPRQGDILLLNTAPRSGHEQTGRRPYIVLSHDIIADTSNVVIVAPITTTSRNYPLYIEITENHKMKTRGKVMLDQIT
TIDYEARNCKFLEKASEKLIEELLTKIRAVFQKV

Download         Length: 345 bp

>T136064 NZ_CP042840:c141548-141204 [Enterococcus sp. DA9]
ATGGTAGATGTTCCAAGACAAGGTGATATTCTTTTATTGAACACAGCTCCCAGATCTGGCCATGAACAGACTGGAAGAAG
ACCTTATATTGTTCTTAGTCACGATATTATTGCAGATACTAGTAATGTGGTTATTGTTGCACCGATAACTACAACTAGTA
GAAATTACCCACTATACATTGAAATAACAGAAAATCATAAAATGAAAACACGCGGAAAAGTTATGTTAGATCAAATCACA
ACAATTGATTATGAAGCCAGGAACTGCAAATTTCTAGAAAAAGCTTCTGAAAAATTAATAGAAGAATTATTAACAAAAAT
ACGAGCTGTATTTCAAAAAGTATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10071.69 Da        Isoelectric Point: 5.2300

>AT136064 WP_002318881.1 NZ_CP042840:c141805-141542 [Enterococcus sp. DA9]
MEMIPIKKWGNSNGLRLPKNLMEFLELKTDDRVSIKSEDVNGKRRLIIEPAISKEEVTIEELFKDYTEGKVNIKIQDLGS
PVGRELW

Download         Length: 264 bp

>AT136064 NZ_CP042840:c141805-141542 [Enterococcus sp. DA9]
ATGGAAATGATACCAATCAAAAAATGGGGAAATAGCAACGGACTTAGATTGCCAAAAAATTTAATGGAATTTCTGGAATT
AAAGACTGATGATCGAGTATCAATCAAAAGCGAAGATGTTAATGGAAAAAGACGATTAATTATCGAACCAGCAATTTCAA
AAGAAGAAGTAACTATTGAAGAGCTTTTCAAAGATTACACAGAGGGCAAAGTAAATATTAAAATTCAGGATTTAGGAAGC
CCAGTAGGGAGAGAACTATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V7YM55

Antitoxin

Source ID Structure
AlphaFold DB A0A6B3Q8V0

References