Detailed information of TA system TA136041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 20641..21194
• Replicon: plasmid pow41
• Accession: NZ_CP042820
• Organism: Octadecabacter sp. SW4

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FTO60_RS16645
• Protein ID: WP_148057206.1
• Coordinates: 20946..21194 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FTO60_RS16640
• Protein ID: WP_172623948.1
• Coordinates: 20641..20892 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FTO60_RS16610	16333..17226	+	894	WP_065334271.1	NAD(P)H-binding protein	-
FTO60_RS16615	17250..17738	+	489	WP_065334266.1	hypothetical protein	-
FTO60_RS16620	17969..19402	+	1434	WP_148057202.1	ISKra4 family transposase	-
FTO60_RS16630	19664..19873	-	210	WP_148057203.1	hypothetical protein	-
FTO60_RS16635	19873..20514	-	642	WP_148057204.1	AAA family ATPase	-
FTO60_RS16640	20641..20892	-	252	WP_172623948.1	BrnA antitoxin family protein	Antitoxin
FTO60_RS16645	20946..21194	-	249	WP_148057206.1	BrnT family toxin	Toxin
FTO60_RS16650	21251..21790	-	540	WP_148057207.1	recombinase family protein	-
FTO60_RS16655	22110..23069	+	960	WP_148057208.1	efflux RND transporter periplasmic adaptor subunit	-
FTO60_RS16660	23083..24618	+	1536	WP_172623942.1	ATP-binding cassette domain-containing protein	-
FTO60_RS16665	24615..24827	+	213	WP_148057210.1	hypothetical protein	-
FTO60_RS16670	24824..25917	+	1094	Protein_25	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108352	108352

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (7-80)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9238.73 Da
• Isoelectric Point: 5.7905

>T136041 WP_148057206.1 NZ_CP042820:c21194-20946 [Octadecabacter sp. SW4]
MIMIVWDEPKRQANLARHGLDFADLDEWFFLDAVTVPAKENRYMAIGRMNDGTIAVVFAVLGTEGVSVISMRPASRKERS
LL

Nucleotide

• Length: 249 bp

>T136041 NZ_CP042820:c21194-20946 [Octadecabacter sp. SW4]
ATGATAATGATTGTCTGGGATGAGCCGAAACGGCAGGCCAACCTTGCACGGCATGGGTTGGATTTTGCTGACCTTGATGA
ATGGTTTTTCCTCGACGCCGTGACGGTCCCTGCAAAGGAAAACCGTTACATGGCTATAGGGCGCATGAACGATGGCACCA
TCGCCGTGGTCTTTGCTGTTCTGGGAACGGAAGGCGTGTCGGTTATCTCCATGCGCCCTGCAAGCCGCAAGGAAAGGAGC
CTGCTATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9046.34 Da
• Isoelectric Point: 10.5623

>AT136041 WP_172623948.1 NZ_CP042820:c20892-20641 [Octadecabacter sp. SW4]
MIASDPDAAEVTDDQIAKAKPFTEAFPALAENMRKNVGGRPRSKNPKVPVSIRLDQDVVAKFKATGPGWQSRINDVLRKS
AVQ

Nucleotide

• Length: 252 bp

>AT136041 NZ_CP042820:c20892-20641 [Octadecabacter sp. SW4]
ATGATTGCCAGCGATCCTGACGCAGCTGAGGTGACAGACGACCAAATCGCCAAGGCAAAGCCATTCACGGAGGCTTTCCC
GGCCCTAGCCGAGAACATGCGCAAAAATGTCGGCGGACGTCCACGGTCCAAGAATCCCAAAGTGCCGGTGTCGATCCGGC
TGGATCAGGATGTGGTCGCAAAGTTCAAAGCGACAGGTCCAGGCTGGCAAAGCCGGATCAACGATGTGCTGCGTAAGTCG
GCGGTTCAGTAA