Detailed information of TA system TA136037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 89793..90376
• Replicon: plasmid unnamed2
• Accession: NZ_CP042810
• Organism: Acetobacter oryzoeni strain B6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A177GCG6
• Locus tag: EOV40_RS14545
• Protein ID: WP_063907590.1
• Coordinates: 89793..90137 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EOV40_RS14550
• Protein ID: WP_128106459.1
• Coordinates: 90134..90376 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOV40_RS14540	85399..89241	+	3843	WP_128106458.1	CHAT domain-containing protein	-
EOV40_RS14545	89793..90137	-	345	WP_063907590.1	endoribonuclease MazF	Toxin
EOV40_RS14550	90134..90376	-	243	WP_128106459.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EOV40_RS14555	90823..91323	-	501	WP_064776424.1	MucR family transcriptional regulator	-
EOV40_RS14560	91963..92454	-	492	WP_020936679.1	type II toxin-antitoxin system YhaV family toxin	-
EOV40_RS14565	92457..92789	-	333	WP_099542213.1	type II toxin-antitoxin system PrlF family antitoxin	-
EOV40_RS14570	92922..93001	+	80	Protein_84	IS5/IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..132758	132758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12504.67 Da
• Isoelectric Point: 10.1798

>T136037 WP_063907590.1 NZ_CP042810:c90137-89793 [Acetobacter oryzoeni]
VSAKAKTVWVPDCGEIVWLEFDPQAGREQAGHRPAVVLSPASYNAKAGMMLCCPTTTKIKGYPFEVALTGKTASVALSDQ
VRSLDWRVRKAKLKGKVLPEELEAIRQRIRLLVG

Nucleotide

• Length: 345 bp

>T136037 NZ_CP042810:c90137-89793 [Acetobacter oryzoeni]
GTGAGTGCCAAAGCAAAGACTGTCTGGGTTCCTGATTGCGGGGAGATTGTCTGGCTGGAATTTGATCCGCAGGCGGGCAG
GGAGCAGGCAGGACACCGGCCTGCTGTTGTGCTGAGTCCTGCAAGTTATAATGCCAAGGCTGGTATGATGCTGTGCTGTC
CGACAACAACCAAGATCAAAGGCTATCCTTTTGAAGTGGCTCTGACTGGAAAAACTGCCAGTGTGGCCTTGTCAGATCAG
GTGAGAAGTCTGGATTGGCGCGTGAGAAAGGCAAAGCTCAAAGGCAAAGTTTTACCCGAAGAGCTTGAGGCCATTCGACA
GCGTATCCGGTTGCTAGTCGGGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8737.89 Da
• Isoelectric Point: 4.4488

>AT136037 WP_128106459.1 NZ_CP042810:c90376-90134 [Acetobacter oryzoeni]
MQGVVRKWGNSAAIRLPAGVLEAVSLKIDQAVDVREEDGRIIIEPVRALPLKLEELVSGITNENRHDEIDFGSSVGGESW

Nucleotide

• Length: 243 bp

>AT136037 NZ_CP042810:c90376-90134 [Acetobacter oryzoeni]
ATGCAGGGCGTTGTCAGGAAATGGGGGAACAGTGCAGCCATTCGTCTTCCTGCGGGTGTGCTTGAAGCCGTCAGTCTGAA
GATTGATCAGGCCGTCGACGTGCGAGAAGAAGATGGGAGGATTATCATAGAACCAGTCAGAGCGTTACCTCTCAAACTGG
AAGAGTTAGTGTCAGGCATTACGAACGAGAACCGCCATGATGAAATTGATTTCGGTTCGTCTGTTGGTGGTGAAAGCTGG
TGA

Structures

Toxin

• AlphaFold DB: A0A177GCG6