Detailed information of TA system TA136036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 224931..225313
• Replicon: plasmid unnamed1
• Accession: NZ_CP042809
• Organism: Acetobacter oryzoeni strain B6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EOV40_RS13995
• Protein ID: WP_081939029.1
• Coordinates: 224931..225086 (-)
• Length: 52 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EOV40_RS14000
• Protein ID: WP_128106376.1
• Coordinates: 225071..225313 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOV40_RS15070	220082..220219	+	138	WP_167506911.1	hypothetical protein	-
EOV40_RS13975	220548..222629	+	2082	WP_128106409.1	NgoFVII family restriction endonuclease	-
EOV40_RS13980	222918..223226	+	309	Protein_227	type II toxin-antitoxin system PemK/MazF family toxin	-
EOV40_RS13985	223276..223830	+	555	Protein_228	cell filamentation protein Fic	-
EOV40_RS13990	223843..224928	-	1086	WP_128106375.1	hypothetical protein	-
EOV40_RS13995	224931..225086	-	156	WP_081939029.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EOV40_RS14000	225071..225313	-	243	WP_128106376.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
EOV40_RS14005	225400..225856	-	457	Protein_232	IS5 family transposase	-
EOV40_RS14010	225985..226491	-	507	WP_128106378.1	rhodanese family protein	-
EOV40_RS14015	226496..226810	-	315	WP_048854868.1	metalloregulator ArsR/SmtB family transcription factor	-
EOV40_RS14020	226940..227080	+	141	Protein_235	PIN domain nuclease	-
EOV40_RS14025	227232..227834	+	603	WP_128106379.1	helix-turn-helix domain-containing protein	-
EOV40_RS14030	227843..229048	-	1206	WP_128106380.1	porin	-
EOV40_RS14035	229035..229334	-	300	WP_128106381.1	hypothetical protein	-
EOV40_RS14040	229538..229837	+	300	Protein_239	ATP-binding protein	-
EOV40_RS14045	229764..229955	+	192	WP_128106382.1	hypothetical protein	-
EOV40_RS14050	229925..230071	+	147	Protein_241	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..246434	246434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-39)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5850.79 Da
• Isoelectric Point: 10.5191

>T136036 WP_081939029.1 NZ_CP042809:c225086-224931 [Acetobacter oryzoeni]
IGRSVSCYKIKRRASGYRLVYQVHEGKLIVLVVAIGRRDANEVYDDATRRV

Nucleotide

• Length: 156 bp

>T136036 NZ_CP055219:3349712-3349885 [Shigella flexneri]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8711.00 Da
• Isoelectric Point: 4.3569

>AT136036 WP_128106376.1 NZ_CP042809:c225313-225071 [Acetobacter oryzoeni]
MASINLCIDDSLKKAAYDRLSELGVTSSDLIRQTVEYVVATGKLPVRRTVVSDDGLELLPVVRERLASPEPSIPVSLDDL

Nucleotide

• Length: 243 bp

>AT136036 NZ_CP042809:c225313-225071 [Acetobacter oryzoeni]
ATGGCTTCAATTAACCTGTGCATCGATGACAGTCTGAAGAAAGCTGCATACGATCGTCTGTCAGAACTCGGCGTGACATC
CTCAGATCTTATCCGCCAGACCGTTGAATATGTCGTCGCTACTGGCAAGCTCCCTGTCCGGCGCACTGTTGTATCGGATG
ACGGTCTGGAGCTTCTTCCCGTCGTTCGAGAGCGTCTTGCCTCGCCTGAACCGTCTATTCCGGTTTCATTGGACGATCTG
TGA