Detailed information of TA system TA136035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 52946..53625
• Replicon: plasmid unnamed1
• Accession: NZ_CP042809
• Organism: Acetobacter oryzoeni strain B6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A5B9GMW7
• Locus tag: EOV40_RS13110
• Protein ID: WP_058988177.1
• Coordinates: 52946..53356 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EOV40_RS13115
• Protein ID: WP_208729365.1
• Coordinates: 53353..53625 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOV40_RS13070	48200..48967	+	768	WP_128106294.1	hypothetical protein	-
EOV40_RS13075	49085..49933	+	849	WP_128106295.1	hypothetical protein	-
EOV40_RS13080	49936..50400	+	465	WP_128106296.1	hypothetical protein	-
EOV40_RS13085	50497..51309	+	813	WP_128106297.1	hypothetical protein	-
EOV40_RS13090	51321..51713	+	393	WP_128106298.1	hypothetical protein	-
EOV40_RS13095	51807..52019	-	213	WP_128106397.1	hypothetical protein	-
EOV40_RS13100	52147..52347	-	201	WP_006560470.1	hypothetical protein	-
EOV40_RS13105	52344..52877	-	534	WP_128106299.1	hypothetical protein	-
EOV40_RS13110	52946..53356	-	411	WP_058988177.1	type II toxin-antitoxin system VapC family toxin	Toxin
EOV40_RS13115	53353..53625	-	273	WP_208729365.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EOV40_RS13120	53696..54112	-	417	WP_086651098.1	hypothetical protein	-
EOV40_RS13125	54109..54546	-	438	WP_128106300.1	plasmid protein	-
EOV40_RS13130	54609..55097	-	489	WP_086651096.1	single-stranded DNA-binding protein	-
EOV40_RS15055	55821..55967	-	147	WP_167506905.1	hypothetical protein	-
EOV40_RS13135	55960..56259	-	300	WP_128106301.1	hypothetical protein	-
EOV40_RS13140	56319..56999	-	681	WP_128106302.1	hypothetical protein	-
EOV40_RS13145	57777..58055	+	279	WP_128106303.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..246434	246434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14787.09 Da
• Isoelectric Point: 5.2057

>T136035 WP_058988177.1 NZ_CP042809:c53356-52946 [Acetobacter oryzoeni]
VSAYLLDTNIISDLVRNPSGPCARRIERIDPGDLCTSIIVAAELRYGCAKKGSAKLLNRVESVLAFVPVLPLDIPADCEY
GGIRAELEAAGQTIGMNDLLIAAHAYTLKATLVTNNTREFMRIRGLKVENWLEDGT

Nucleotide

• Length: 411 bp

>T136035 NZ_CP042809:c53356-52946 [Acetobacter oryzoeni]
GTGAGCGCCTATCTCCTTGATACCAATATCATCAGTGATCTTGTCCGAAATCCTTCCGGCCCGTGCGCGCGTCGCATCGA
ACGTATCGATCCCGGCGACCTTTGTACCAGCATTATTGTGGCCGCTGAGTTACGATATGGATGTGCTAAAAAAGGCTCTG
CAAAGCTGTTAAACCGGGTTGAAAGCGTGCTGGCGTTTGTCCCCGTGCTGCCTCTCGATATTCCTGCCGACTGTGAATAC
GGTGGCATCCGCGCCGAACTCGAAGCCGCAGGGCAGACGATCGGCATGAATGATCTGCTCATCGCGGCTCACGCCTACAC
CCTCAAGGCGACCCTGGTGACGAACAACACACGTGAGTTTATGCGCATCCGAGGCTTGAAGGTTGAAAACTGGCTGGAGG
ATGGGACGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10442.11 Da
• Isoelectric Point: 4.8660

>AT136035 WP_208729365.1 NZ_CP042809:c53625-53353 [Acetobacter oryzoeni]
MPVPKVTPRPREVKLFWNNRSQAVRIPVEFQMPGDRVLIRRDGDKLVLEPVKTPSTLKELLMAWREEPQLAPEDDFPDIQ
DVAARPEDIL

Nucleotide

• Length: 273 bp

>AT136035 NZ_CP042809:c53625-53353 [Acetobacter oryzoeni]
ATGCCAGTTCCTAAAGTGACTCCCCGACCACGCGAAGTAAAACTGTTCTGGAACAACCGCAGTCAGGCAGTGCGGATTCC
GGTTGAATTTCAAATGCCAGGAGACCGGGTGCTGATCCGCCGTGACGGTGACAAGCTCGTTCTGGAGCCTGTGAAAACCC
CTTCCACTCTGAAGGAGCTTCTCATGGCATGGCGCGAAGAACCGCAGCTGGCCCCAGAAGACGATTTTCCCGATATTCAG
GACGTAGCCGCCAGACCGGAAGATATTCTGTGA

Structures

Toxin

• AlphaFold DB: A0A5B9GMW7