Detailed information of TA system TA136016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2227621..2228135
• Replicon: chromosome
• Accession: NZ_CP042650
• Organism: Staphylococcus aureus strain X22

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: FTD94_RS11395
• Protein ID: WP_000581792.1
• Coordinates: 2227621..2227830 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: FTD94_RS11400
• Protein ID: WP_044290684.1
• Coordinates: 2227842..2228135 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FTD94_RS11375	2223129..2224487	-	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
FTD94_RS11380	2224502..2225572	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
FTD94_RS11385	2225890..2227092	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
FTD94_RS11390	2227356..2227493	-	138	WP_000828354.1	hypothetical protein	-
FTD94_RS11395	2227621..2227830	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
FTD94_RS11400	2227842..2228135	-	294	WP_044290684.1	copper-sensing transcriptional repressor CsoR	Antitoxin
FTD94_RS11405	2228301..2229785	+	1485	WP_000571549.1	cardiolipin synthase	-
FTD94_RS11410	2229813..2230460	+	648	WP_001187629.1	HD domain-containing protein	-
FTD94_RS11420	2230896..2231768	-	873	WP_000725802.1	membrane protein insertase YidC	-
FTD94_RS11425	2231854..2232495	-	642	WP_044290685.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T136016 WP_000581792.1 NZ_CP042650:c2227830-2227621 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T136016 NZ_CP042650:c2227830-2227621 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11179.79 Da
• Isoelectric Point: 6.2243

>AT136016 WP_044290684.1 NZ_CP042650:c2228135-2227842 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIINKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT136016 NZ_CP042650:c2228135-2227842 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATAAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1