Detailed information of TA system TA136015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2207876..2208405
• Replicon: chromosome
• Accession: NZ_CP042650
• Organism: Staphylococcus aureus strain X22

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FTD94_RS11305
• Protein ID: WP_000621175.1
• Coordinates: 2207876..2208238 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FTD94_RS11310
• Protein ID: WP_000948331.1
• Coordinates: 2208235..2208405 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FTD94_RS11285	2204855..2205625	-	771	WP_147091803.1	RNA polymerase sigma factor SigB	-
FTD94_RS11290	2205600..2206079	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
FTD94_RS11295	2206081..2206407	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FTD94_RS11300	2206526..2207527	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FTD94_RS11305	2207876..2208238	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FTD94_RS11310	2208235..2208405	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FTD94_RS11315	2208490..2209638	-	1149	WP_001281145.1	alanine racemase	-
FTD94_RS11320	2209704..2210063	-	360	WP_000581202.1	holo-ACP synthase	-
FTD94_RS11325	2210067..2210558	-	492	WP_078103616.1	PH domain-containing protein	-
FTD94_RS11330	2210545..2212128	-	1584	WP_001294626.1	PH domain-containing protein	-
FTD94_RS11335	2212121..2212600	-	480	WP_147091804.1	hypothetical protein	-
FTD94_RS11340	2212809..2213369	-	561	WP_044290681.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T136015 WP_000621175.1 NZ_CP042650:c2208238-2207876 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T136015 NZ_CP042650:c2208238-2207876 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT136015 WP_000948331.1 NZ_CP042650:c2208405-2208235 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT136015 NZ_CP055216:c2497151-2496708 [Raoultella ornithinolytica]
ATGAAAACCTGGGTTCCTGCAGAGCAACCTGCCGATAATGCGCTGTGGCGCTATGCCGGGCTACCGGCAAATCGGGGTAT
GCAGTTGATTGAGCTGCTGAGTATGGGATTACCGGTCAGCATTCTGGATAATATTCATGAGTGGACGGAAATGTCGAAAA
CCGACATTCTGCGCATCACCGGGATTAATGAGCGCAACGTTGCCCGGCGCAAGAGCGCGGGCGGCACGTTGACGCCAGGC
GAAAGCGAACGTGTGGCGCGTTTTGTCAGGGTCCTGGATACCGCCGTCGACTATTTTGGCAGCAAGCAGGATGCCTACGA
CTGGCTGCAGTCGCCGGTCAGAGGACTGGGCAATGTCGCCCCCATCGATCTGATTGCTACCGAGAGCGGCGCGCTGGAAG
TAACAGACCTGATTGGTCGCCTGGAACACGGCGTCTTTGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1